Immunoglobulin synthesis and secretion. V. Incorporation of leucine and glucosamine into immunoglobulin on free and bound polyribosomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 66 ·No. 4 ·1970-08-00 ·Pages 1183-9

Sherr CJ, Uhr JW

Abstract

Mouse myeloma cells pulse-labeled in vitro with (3)H-leucine or (3)H-glucosamine were fractionated on sucrose gradients, and membrane-bound and free polyribosomes were separated. Nascent polypeptides released from polyribosomes were precipitated with antiserum specific for mouse immunoglobulin. The results indicate that immunoglobulin is synthesized preferentially by bound polyribosomes.

MeSH Terms

Animals Cell Line Centrifugation, Density Gradient Chromatography, Gel Glucosamine/metabolism Immune Sera Immunoglobulin G/biosynthesis Leucine/metabolism Mice Precipitin Tests Rabbits Ribosomes/metabolism Sarcoma Tritium

Chemicals

Immune Sera Immunoglobulin G Tritium Leucine Glucosamine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sherr C J

Uhr J W

References (28)

28 references, click to expand

Studies on the protein-synthesizing activity of the ribosomes of rat liver. The activity of free polysomes.
Biochem J. 1965 Nov;97(2):422-31 PMID: 5880015
Synthesis and transfer of amylase in pigeon pancreatic micromosomes.
J Biol Chem. 1966 Mar 10;241(5):1150-8 PMID: 5933873
Biosynthesis of glycoprotein by liver. The incorporation in vivo of 14C-glucosamine into protein-bound hexosamine and sialic acid of rat liver subcellular fractions.
J Biol Chem. 1966 Nov 25;241(22):5408-18 PMID: 5954806
On the biological role of glycoproteins.
J Theor Biol. 1966 Jan;10(1):89-113 PMID: 5337048
Polyribosomal synthesis and assembly of the H and L chains of gamma globulin.
Proc Natl Acad Sci U S A. 1966 Jul;56(1):216-21 PMID: 4165344
The synthesis and storage of protein by isolated lymphoid cells, examined by autoradiography with the electron microscope.
Am J Anat. 1966 Nov;119(3):375-403 PMID: 5972730
Biosynthesis of immunoglobulins: the separate classes of polyribosomes synthesizing heavy and light chains.
J Mol Biol. 1967 Jan 28;23(2):201-16 PMID: 6031716
Studies on free and membrane-bound ribosomes in rat liver. II. Interaction of ribosomes and membranes.
J Mol Biol. 1967 Jun 14;26(2):293-301 PMID: 6034776
Ribosomes in rat liver: an estimate of the percentage of free and membrane-bound ribosomes interacting with messenger RNA in vivo.
J Mol Biol. 1967 Sep 28;28(3):539-42 PMID: 6052649
Ultrastructural localization of antibody in differentiating plasma cells.
J Exp Med. 1968 Jan 1;127(1):109-18 PMID: 5635036
Early labeling with glucosamine-C14 of granular and agranular endoplasmic reticulum and free ribosomes from rat liver.
Arch Biochem Biophys. 1968 Jun;125(3):715-20 PMID: 5671038
Direct evidence for albumin biosynthesis by membrane bound polysomes in rat liver.
Biochem Biophys Res Commun. 1968 Oct 10;33(1):55-60 PMID: 5696506
Immunoglobulin assembly in a mouse myeloma.
Proc Natl Acad Sci U S A. 1968 Jun;60(2):683-90 PMID: 4178281
Preferential synthesis of ferritin and albumin by different populations of liver polysomes.
Science. 1969 May 2;164(3879):584-5 PMID: 5781696
Biosynthesis of serum proteins and ferritin by free and attached ribosomes of rat liver.
J Biol Chem. 1969 Aug 25;244(16):4308-15 PMID: 5806579
Glycoprotein synthesis by membraneound ribosomes and smooth membranes in kidney.
Biochim Biophys Acta. 1969 Sep 17;190(1):154-60 PMID: 5822846
Glycoproteins: their biochemistry, biology and role in human disease (first of two parts).
N Engl J Med. 1969 Oct 30;281(18):991-1001 contd PMID: 4186433
In vitro synthesis of different categories of specific protein by membrane-bound and free ribosomes.
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1370-6 PMID: 5260940
Immunoglobulin synthesis and secretion. 3. Incorporation of glucosamine into immunoglobulin on polyribosomes.
Proc Natl Acad Sci U S A. 1969 Sep;64(1):381-7 PMID: 5263020
Immunoglobulin synthesis and secretion, IV. Sites of incorporation of sugars as determined by subcellular fractionation.
Proc Natl Acad Sci U S A. 1970 Jul;66(3):952-8 PMID: 5269256
Synthesis of immunoglobulin heavy and light chains by the free ribosomes of a mouse plasma cell tumor.
Proc Natl Acad Sci U S A. 1970 Jun;66(2):425-32 PMID: 4194867
Controlled proteolysis of nascent polypeptides in rat liver cell fractions. II. Location of the polypeptides in rough microsomes.
J Cell Biol. 1970 Apr;45(1):146-57 PMID: 5458993
Immunoglobulin synthesis and secretion. I. Biosynthetic studies of the addition of the carbohydrate moieties.
J Cell Biol. 1970 Jul;46(1):42-51 PMID: 5459011
Immunoglobulin synthesis and secretion. II. Radioautographic studies of sites of addition of carbohydrate moieties and intracellular transport.
J Cell Biol. 1970 Jul;46(1):52-63 PMID: 5460463
The biosynthesis of rat serum albumin. I. Properties of rat albumin and its occurrence in liver cell fractions.
J Biol Chem. 1962 Apr;237:1181-5 PMID: 14485748
A cytochemical study on the pancreas of the guinea pig. 5. In vivo incorporation of leucine-1-C14 into the chymotrypsinogen of various cell fractions.
J Biophys Biochem Cytol. 1960 Jul;7:619-30 PMID: 14446510
SYNTHESIS OF SERUM PROTEINS BY A CELL-FREE SYSTEM FROM RAT LIVER.
Proc Natl Acad Sci U S A. 1965 Mar;53:619-22 PMID: 14338242
Studies on the synthesis o serum albumin by the isolated microsome fraction from rat liver.
Biochem J. 1960 Jan;74:107-17 PMID: 13807296

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.66.4.1183

Published

1970-08-00

Pages

1183-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC335804

Subset

External Links

PubMed source DOI: 10.1073/pnas.66.4.1183 PMC Full Text View journal details

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Immunoglobulin synthesis and secretion. V. Incorporation of leucine and glucosamine into immunoglobulin on free and bound polyribosomes.

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