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PMID: 620100 Published · ppublish English Journal Article

Phospholipase A2 activity of guinea-pig isolated perfused lungs: stimulation, and inhibition by anti-inflammatory steroids.

British journal of pharmacology ·Vol. 62 ·No. 1 ·1978-01-00 ·Pages 79-89

Blackwell GJ, Flower RJ, Nijkamp FP, Vane JR

Abstract

1 A simple double-isotope assay for phospholipase A2 activity of perfused organs is described; Guinea-pig lungs perfused through the pulmonary circulation exhibit a low background enzyme activity. This activity is blocked by dexamethasone, betamethasone and hydrocortisone, mepacrine, procaine or chlorpromazine. Aspirin and indomethacin are without effect. 3 Mechanical trauma, antigen challenge or injections of bradykinin, rabbit aorta contracting substance-releasing factor (RCS-RF) or histamine increase "basal" phospholipase activity. The effect of these agents, except that of bradykinin, is blocked by dexamethasone or mepacrine. 4 The blocking effect of steroids is cumulative and dose-dependent. They do not work in cell-free systems. Inhibition by mepacrine is rapid and is effective in cell-free lung homogenates. 5 It is suggested that agents which liberate prostaglandin endoperoxides and thromboxane A2 from perfused lungs do so by activating phospholipase A2.

MeSH Terms
Animals Anti-Inflammatory Agents/pharmacology Glucocorticoids/pharmacology Guinea Pigs Hydrolysis In Vitro Techniques Lung/enzymology Male Perfusion Phosphatidylcholines/pharmacology Phospholipases/antagonists & inhibitors,metabolism Time Factors
Chemicals
Anti-Inflammatory Agents Glucocorticoids Phosphatidylcholines Phospholipases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Blackwell G J
Flower R J
Nijkamp F P
Vane J R
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27 references, click to expand
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Article Info
Journal
British journal of pharmacology
Abbr.
Br J Pharmacol
ISSN
0007-1188
DOI
10.1111/j.1476-5381.1978.tb07009.x
Published
1978-01-00
Pages
79-89
Language
English
Region
England
NLM ID
7502536
PMCID
PMC1667789
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1476-5381.1978.tb07009.x PMC Full Text View journal details
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