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PMID: 827290 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Trypsin-induced phospholipase activity in human platelets.

The Biochemical journal ·Vol. 160 ·No. 2 ·1976-11-15 ·Pages 405-8

Pickett WC, Jesse RL, Cohen P

Abstract

Trypsin mediates a release of arachidonic acid with resultant increase in O2 consumption (a reflection of cyclo-oxygenase activity) by whole human platelets that is similar to thrombin's effect on these cells. The trypsin and thrombin effects can be differentiated in two ways: (1) at saturating concentrations the measured effects of trypsin greatly exceed those of thrombin; (2) EGTA [ethanedioxybis(ethylamine)-NNN'N'-tera-acetate] augments the effect of thrombin but not of trypsin. Thus trypsin and thrombin probably act at different loci in the pathway that induces phospholipase activity in human platelets.

MeSH Terms
Arachidonic Acids/metabolism Blood Platelets/metabolism Egtazic Acid/pharmacology Humans Oxygen Consumption Phospholipases/metabolism Platelet Aggregation/drug effects Thrombin/antagonists & inhibitors,pharmacology Trypsin/pharmacology
Chemicals
Arachidonic Acids Egtazic Acid Phospholipases Trypsin Thrombin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pickett W C
Jesse R L
Cohen P
References (9)
9 references, click to expand
  1. Reversible alterations in platelet morphology produced by anticoagulants and by cold.
    Blood. 1954 Jun;9(6):602-8 PMID: 13160109
  2. An improved method for isolating alpha granules and mitochondria from human platelets.
    J Cell Biol. 1974 Feb;60(2):507-19 PMID: 4360293
  3. Patterns of fatty acid release from endogenous substrates by human platelet homogenates and membranes.
    J Biol Chem. 1975 Dec 25;250(24):9342-7 PMID: 394
  4. The binding of thrombin to the surface of human platelets.
    J Biol Chem. 1974 Apr 25;249(8):2646-51 PMID: 4856653
  5. [Action of blood platelets on plasma prophospholipase].
    Biochimie. 1972;54(2):257-60 PMID: 5086549
  6. Changes in platelet volume produced by temperature, metabolic inhibitors, and aggregating agents.
    Proc Soc Exp Biol Med. 1965 Nov;120(2):296-301 PMID: 4955168
  7. Influence of medium tonicity on respiration by suspensions of human platelets.
    Am J Physiol. 1976 Jul;231(1):185-90 PMID: 961859
  8. Mechanism of the thrombin-mediated burst in oxygen consumption by human platelets.
    J Biol Chem. 1976 Apr 25;251(8):2536-8 PMID: 1262333
  9. Purification and properties of phospholipase A and its zymogen from porcine pancreas.
    Bull Soc Chim Biol (Paris). 1968 Dec;50(9):1383 PMID: 5704365
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1600405
Published
1976-11-15
Pages
405-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1164248
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1600405 PMC Full Text View journal details
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