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PMID: 6200831 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Map locations of five transcripts homologous to TR-DNA in tobacco and sunflower crown gall tumors.

Nucleic acids research ·Vol. 12 ·No. 5 ·1984-03-12 ·Pages 2391-406

Winter JA, Wright RL, Gurley WB

Abstract

Polyadenylated RNA from two octopine type tumor lines (E1, PSCG-15955) was analyzed by RNA blot hybridization and shown to contain five major transcripts homologous to TR DNA. In tobacco E1 tissue, the molecular weights of the TR homologous RNAs are 1.65 kb, 1.55 kb, 1.45 kb, 1.05 kb, and 0.78 kb. The 5' - and 3'-termini of each of the five E1 tumor transcripts were determined by S1 nuclease hybrid protection mapping. The polarity of transcription for the 0.78, 1.05 and 1.65 kb RNAs is from left to right, while the 1.55 and 1.45 kb RNAs are transcribed from right to left. Although we see the same size transcripts in sunflower PSCG-15955 tissue (with the exception of a 0.89 kb RNA in place of the 1.55 kb transcript of E1) there is an apparent difference in the relative abundance of the various RNAs between the two tumor lines.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA, Neoplasm/genetics Nucleic Acid Hybridization Plant Tumors Plants/genetics Plants, Toxic Poly A/genetics RNA/genetics RNA, Messenger Rhizobium/genetics Species Specificity Tobacco/genetics Transcription, Genetic
Chemicals
DNA, Neoplasm RNA, Messenger Poly A RNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Winter J A
Wright R L
Gurley W B
References (29)
29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.5.2391
Published
1984-03-12
Pages
2391-406
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318670
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/12.5.2391 PMC Full Text View journal details
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