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PMID: 6175956 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

T-DNA of pTi-15955 from Agrobacterium tumefaciens is transcribed into a minimum of seven polyadenylated RNAs in a sunflower crown gall tumor.

Nucleic acids research ·Vol. 10 ·No. 5 ·1982-03-11 ·Pages 1679-89

Murai N, Kemp JD

Abstract

Northern blot hybridization analysis of polysomal polyadenylated RNA isolated from sunflower crown gall tumor PSCG-15955 demonstrated that a minimum of seven RNAs were transcribed from T-DNA of pTi-15955 from Agrobacterium tumefaciens. The sizes of the T-DNA transcripts were 1.8, 1.6, 1.5, 1.1, 1.0 kilo bases (kb) and two transcripts of 0.8 kb long. The relative abundance of these polyadenylated RNAs varied greatly, the 1.0 kb RNA being the most abundant and the 1.6 kb RNA being the least abundant. Assignment of map locations of the seven polyadenylated RNAs indicated that the conserved region of T-DNA which may play a central role in tumorigenesis contained four RNAs of 1.8, 1.1, 0.8(a) and a portion of 0.8(b) kb long.

MeSH Terms
DNA Restriction Enzymes DNA, Bacterial/genetics DNA, Recombinant/metabolism Nucleic Acid Hybridization Plant Tumors Plasmids Poly A/genetics Polyribosomes/metabolism RNA/genetics RNA, Messenger Rhizobium/genetics Transcription, Genetic
Chemicals
DNA, Bacterial DNA, Recombinant RNA, Messenger Poly A RNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Murai N
Kemp J D
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27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.5.1679
Published
1982-03-11
Pages
1679-89
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320558
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.5.1679 PMC Full Text View journal details
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