Home LiteratureArticle Details
PMID: 6179090 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of cDNA clones coding for rat tyrosine hydroxylase antigen.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 12 ·1982-06-00 ·Pages 3881-5

Lamouroux A, Faucon Biguet N, Samolyk D, Privat A, Salomon JC, Pujol JF, Mallet J

Abstract

Five recombinant DNA plasmids have been constructed that contain structural gene sequences for rat tyrosine hydroxylase [TyrOHase; tyrosine 3-monooxygenase; L-tyrosine, tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating), EC 1.14.16.2]. Rat pheochromocytoma PC 12 cell line, which contains relatively high levels of catecholamine-synthesizing enzymes, was used to purify RNA. TyrOHase cDNA clones were identified by screening 350 cDNA clones constructed from partially purified TyrOHase mRNA. A rapid and powerful screening of the recombinant clones by differential colony hybridization was possible because TyrOHase is a tissue-specific protein. The final selection relied on the ability of cDNA inserts to hybridize specifically to TyrOHase mRNA as judged by cell-free translation and immunoprecipitation. Blot hybridization analysis of polyadenylylated RNA from PC 12 cells indicated a major mRNA species of 1.9 kilobases. A species of the same size was identified from a human pheochromocytoma tumor, indicating a crossreactivity between rat TyrOHase cDNA and human TyrOHase mRNA.

MeSH Terms
Animals Cells, Cultured Cloning, Molecular DNA/genetics Epitopes Humans Pheochromocytoma RNA, Messenger/genetics Rats Tyrosine 3-Monooxygenase/genetics,immunology
Chemicals
Epitopes RNA, Messenger DNA Tyrosine 3-Monooxygenase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lamouroux A
Faucon Biguet N
Samolyk D
Privat A
Salomon J C
Pujol J F
Mallet J
References (33)
33 references, click to expand
  1. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  4. Immunochemical demonstration of increased accumulation of tyrosine hydroxylase protein in sympathetic ganglia and adrenal medulla elicited by reserpine.
    Proc Natl Acad Sci U S A. 1973 Oct;70(10):2767-71 PMID: 4147606
  5. Trans-synaptic enzyme induction.
    Life Sci. 1974 Jan 16;14(2):223-35 PMID: 4149845
  6. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  7. Nerve growth factor induces volume increase and enhances tyrosine hydroxylase synthesis in chemically axotomized sympathetic ganglia of newborn rats.
    Proc Natl Acad Sci U S A. 1975 Feb;72(2):595-9 PMID: 235759
  8. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  9. Preparation of pancreatic mRNA: cell-free translation of an insulin-immunoreactive polypeptide.
    Nucleic Acids Res. 1976 Feb;3(2):381-91 PMID: 1257052
  10. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2424-8 PMID: 1065897
  11. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
  12. Effect of glucocorticoids on nerve growth factor-mediated enzyme induction in organ cultures of rat sympathetic ganglia: enchanced response and reduced time requirement to initiate enzyme induction.
    J Neurochem. 1977 Jul;29(1):69-75 PMID: 18557
  13. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  14. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  15. Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.
    J Biol Chem. 1978 Apr 10;253(7):2483-95 PMID: 75887
  16. Selective enzyme induction in a nerve growth factor-responsive pheochromocytoma cell line (PC 12).
    Brain Res. 1978 Oct 6;154(1):186-90 PMID: 81091
  17. Nerve growth factor-mediated induction of tyrosine hydroxylase in a clonal pheochromocytoma cell line.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4587-90 PMID: 30087
  18. Direct phosphorylation of brain tyrosine hydroxylase by cyclic AMP-dependent protein kinase: mechanism of enzyme activation.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4744-8 PMID: 33381
  19. Cell-free synthesis and membrane insertion of mouse H-2Dd histocompatibility antigen and beta 2-microglobulin.
    Cell. 1979 Aug;17(4):759-69 PMID: 90552
  20. Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927-31 PMID: 291909
  21. Filter replicas and permanent collections of recombinant DNA plasmids.
    Nucleic Acids Res. 1979 Dec 20;7(8):2115-36 PMID: 118435
  22. Addition of homopolymers to the 3'-ends of duplex DNA with terminal transferase.
    Methods Enzymol. 1979;68:41-50 PMID: 542126
  23. Maximizing gene expression on a plasmid using recombination in vitro.
    Methods Enzymol. 1979;68:473-82 PMID: 232222
  24. Purification and characterization of tyrosine hydroxylase from a clonal pheochromocytoma cell line.
    Mol Pharmacol. 1980 Jan;17(1):79-85 PMID: 6104286
  25. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
    Eur J Biochem. 1980 Jun;107(2):303-14 PMID: 6772444
  26. The ontogeny of the neural crest in avian embryo chimaeras.
    Nature. 1980 Aug 14;286(5774):663-9 PMID: 6106161
  27. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  28. Molecular cloning of a human histocompatibility antigen cDNA fragment.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):6081-5 PMID: 6934534
  29. Developmental regulation of neurotransmitter phenotype.
    Curr Top Dev Biol. 1980;15 Pt 1:27-40 PMID: 6108833
  30. [In vitro synthesis of tyrosine hydroxylase].
    C R Seances Acad Sci III. 1981 Feb 9;292(6):455-60 PMID: 6163570
  31. Translation of tyrosine hydroxylase from poly(A)-mRNA in pheochromocytoma cells is enhanced by dexamethasone.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):1269-73 PMID: 6112744
  32. Importance of fixation in immunohistochemistry: use of formaldehyde solutions at variable pH for the localization of tyrosine hydroxylase.
    J Histochem Cytochem. 1981 Jul;29(7):844-50 PMID: 6167611
  33. Dopaminergic dendrites in the pars reticulata of the rat substantia nigra and their striatal input. Combined immunocytochemical localization of tyrosine hydroxylase and anterograde degeneration.
    Neuroscience. 1981;6(11):2125-39 PMID: 6120482
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.12.3881
Published
1982-06-00
Pages
3881-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346532
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.12.3881 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2