Immunochemical demonstration of increased accumulation of tyrosine hydroxylase protein in sympathetic ganglia and adrenal medulla elicited by reserpine.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 10 ·1973-10-00 ·Pages 2767-71

Joh TH, Geghman C, Reis D

Abstract

Chronic administration of reserpine to rats increases, in sympathetic ganglia and adrenal medulla, the activity of tyrosine hydroxylase (EC 1.14.3.x), the enzyme catalyzing the rate-limiting step in the biosynthesis of catecholamines. Immunochemical titration of the enzyme in both adrenal gland and innervated superior cervical ganglia demonstrates that enhanced enzyme activity is entirely attributable to accumulation of more specific enzyme protein and not activation of preexistent enzyme molecules.

MeSH Terms

Adrenal Medulla/enzymology Animals Antibody Specificity Antigen-Antibody Reactions Cattle Denervation Enzyme Activation/drug effects Ganglia, Autonomic/enzymology Immunoassay Immunoelectrophoresis Rabbits/immunology Rats Reserpine/pharmacology Tyrosine 3-Monooxygenase/analysis Vagotomy

Chemicals

Reserpine Tyrosine 3-Monooxygenase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Joh T H

Geghman C

Reis D

References (22)

22 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.10.2767

Published

1973-10-00

Pages

2767-71

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC427105

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.10.2767 PMC Full Text View journal details

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Immunochemical demonstration of increased accumulation of tyrosine hydroxylase protein in sympathetic ganglia and adrenal medulla elicited by reserpine.

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