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PMID: 30087 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nerve growth factor-mediated induction of tyrosine hydroxylase in a clonal pheochromocytoma cell line.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 9 ·1978-09-00 ·Pages 4587-90

Goodman R, Herschman HR

Abstract

We have established a clonal cell line, PC-G2, from an experimentally induced rat pheochromocytoma. Administration of nerve growth factor to PC-G2 causes a 4- to 8-fold induction in the specific activity of tyrosine hydroxylase [tyrosine 3-monooxygenase; L-tyrosine,tetrahydropteridine:oxygen oxidoreductase(3-hydroxylating); EC 1.14.16.2]. The response is elicited in a dose-dependent fashion, at concentrations above 0.1 microgram/ml. Antiserum to nerve growth factor inhibited the induction of tyrosine hydroxylase. Dexamethasone enhances the nerve growth factor-mediated elevation of tyrosine hydroxylase. After 3--4 days of exposure to nerve growth factor the maximal induction of tyrosine hydroxylase is seen, although a significant increase can be observed after 24 hr. In contrast to the PC-12 cell line (derived from the same tumor), in which neurite outgrowth occurs in response to nerve growth factor, there is no morphological change or alteration in growth rate of PC-G2 cells after exposure to nerve growth factor.

MeSH Terms
Cell Line Dexamethasone/pharmacology Drug Synergism Enzyme Induction/drug effects Kinetics Neoplasms, Experimental/enzymology Nerve Growth Factors/pharmacology Pheochromocytoma/enzymology Tyrosine 3-Monooxygenase/biosynthesis
Chemicals
Nerve Growth Factors Dexamethasone Tyrosine 3-Monooxygenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goodman R
Herschman H R
References (21)
21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.9.4587
Published
1978-09-00
Pages
4587-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336162
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.9.4587 PMC Full Text View journal details
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