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PMID: 6094502 Published · ppublish English Journal Article

Control of F plasmid replication by a host gene: evidence for interaction of the mafA gene product of Escherichia coli with the mini-F incC region.

Journal of bacteriology ·Vol. 160 ·No. 3 ·1984-12-00 ·Pages 1130-6

Wada C, Yura T

Abstract

Replication of F (including mini-F) and some related plasmids is known to be specifically inhibited in mafA mutants of Escherichia coli K-12. We have now isolated and characterized mini-F mutants that can overcome the replication inhibition. Such plasmids, designated pom (permissive on maf), were obtained spontaneously or after mutagenesis with hydroxylamine or by transposon (Tn3) insertion. In addition to their ability to replicate in mafA mutant bacteria, the pom mutant plasmids exhibit an increased copy number and resistance to "curing" by acridine dye in the mafA+ host. In agreement with these results, Tn3-induced pom mutants were found to carry Tn3 inserted at the incC region of mini-F DNA, known to be involved in incompatibility, control of copy number, and sensitivity to acridine dye. Furthermore, three of the seven mini-F plasmids tested that carry Tn3 within the tandem repeat sequences of the incC region (previously isolated by other workers) exhibit all the phenotypes of pom plasmids, the ability to replicate in the mafA strain, and high copy number and acridine resistance in the mafA+ strain. The rest of the plasmids that contain Tn3 just outside the tandem repeats remain wild type in all these properties. These results strongly suggest that the putative mafA gene product of host bacteria controls mini-F replication through interaction with the incC region.

MeSH Terms
Acridine Orange/pharmacology Base Sequence DNA Replication DNA Restriction Enzymes DNA Transposable Elements Escherichia coli/genetics F Factor/drug effects Genes Genes, Bacterial Hydroxylamine Hydroxylamines/pharmacology Mutation Phenotype
Chemicals
DNA Transposable Elements Hydroxylamines Hydroxylamine DNA Restriction Enzymes Acridine Orange
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wada C
Yura T
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37 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.160.3.1130-1136.1984
Published
1984-12-00
Pages
1130-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215830
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.160.3.1130-1136.1984 PMC Full Text View journal details
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