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PMID: 6093056 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The complete nucleotide sequence of a common cold virus: human rhinovirus 14.

Nucleic acids research ·Vol. 12 ·No. 20 ·1984-10-25 ·Pages 7859-75

Stanway G, Hughes PJ, Mountford RC, Minor PD, Almond JW

Abstract

The complete nucleotide sequence of the single-stranded RNA genome of human rhinovirus 14, one of the causative agents of the common cold, has been determined from cDNA cloned in E. coli. The genome is typical of the picornaviridae family, comprising a 5' non-coding region of 624 nucleotides, a long open reading frame of 6537 nucleotides (90.8% of the genome) and a 3' non-coding region of 47 nucleotides. Comparison of the nucleotide sequence and the predicted amino acid sequence with those of the polioviruses reveals a surprising degree of homology which may allow recognition of regions of antigenic importance and prediction of the virus polyprotein cleavage sites. The results presented here imply a closer genetic relationship between the rhinovirus and enterovirus genera than previously suspected.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/isolation & purification DNA Restriction Enzymes Genes, Viral HeLa Cells Humans RNA, Viral/genetics Rhinovirus/genetics Species Specificity
Chemicals
RNA, Viral DNA DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Stanway G
Hughes P J
Mountford R C
Minor P D
Almond J W
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34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.20.7859
Published
1984-10-25
Pages
7859-75
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320205
Subset
IM
Databases
GENBANK
K02121, X01087
External Links
PubMed source DOI: 10.1093/nar/12.20.7859 PMC Full Text View journal details
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