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PMID: 6324136 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The nucleotide and deduced amino acid sequences of the encephalomyocarditis viral polyprotein coding region.

Nucleic acids research ·Vol. 12 ·No. 6 ·1984-03-26 ·Pages 2969-85

Palmenberg AC, Kirby EM, Janda MR, Drake NL, Duke GM, Potratz KF, Collett MS

Abstract

The nucleotide sequence of 7200 bases of encephalomyocarditis (EMC) viral RNA, including the complete polyprotein-coding region, was determined. The polyprotein is encoded within a unique translational reading frame, 6870 bases in length. Protein synthesis begins with the sequence Met-Ala-Thr, and ends with the sequence Leu-Phe-Trp, 126 bases from the 3' end of the RNA. Viral capsid and noncapsid proteins were aligned with the deduced amino acid sequence of the polyprotein. The proteolytic processing map follows the standard 4-3-4 picornaviral pattern except for a short leader peptide (8 kd), which precedes the capsid proteins. Identification of the proteolytic cleavage sites showed that EMC viral protease, p22, has cleavage specificity for gln-gly or gln-ser sequences with adjacent proline residues. The cleavage specificity of the host-coded protease(s) includes both tyr-pro and gln-gly sequences.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Codon Coliphages/genetics Encephalomyocarditis virus/genetics Escherichia coli/genetics Genes Genes, Viral Plasmids RNA, Viral/genetics Viral Proteins/genetics
Chemicals
Codon RNA, Viral Viral Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Palmenberg A C
Kirby E M
Janda M R
Drake N L
Duke G M
Potratz K F
Collett M S
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44 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.6.2969
Published
1984-03-26
Pages
2969-85
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318719
Subset
IM
Grants
NIAID NIH HHS · AI 17331 · United States
Databases
GENBANK
X00463
External Links
PubMed source DOI: 10.1093/nar/12.6.2969 PMC Full Text View journal details
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