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PMID: 6331350 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of the genomes of poliovirus type 3 strains by the cDNA: RNA hybrid method.

Archives of virology ·Vol. 81 ·No. 1-2 ·1984-00-00 ·Pages 67-78

Stanway G, Mountford RC, Cox SD, Schild GC, Minor PD, Almond JW

Abstract

The genomes of two neurovirulent strains of poliovirus type 3, wild type P3/Leon/37 and a vaccine revertant P3/119/70, have been cloned in E. coli. The cDNA: RNA hybrid method used was efficient and may have wide applicability for cDNA cloning. Overlapping clones spanning the entire genome were obtained for each strain. These have been used to produce full-length DNA copies of the two genomes each within a single plasmid.

MeSH Terms
Base Sequence Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Escherichia coli/genetics Genes, Viral Nucleic Acid Hybridization Plasmids Poliovirus/genetics Species Specificity
Chemicals
DNA DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Stanway G
Mountford R C
Cox S D
Schild G C
Minor P D
Almond J W
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24 references, click to expand
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Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01309297
Published
1984-00-00
Pages
67-78
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01309297 View journal details
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