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PMID: 3951989 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Moderately repeated DNA sequences specific for the short arm of the human Y chromosome are present in XX males and reduced in copy number in an XY female.

Nucleic acids research ·Vol. 14 ·No. 3 ·1986-02-11 ·Pages 1325-40

Müller U, Lalande M, Donlon T, Latt SA

Abstract

Four DNA sequences specific for the Y chromosome were isolated from a recombinant phage library constructed from flow sorted human Y chromosomes. Two of these sequences were moderately repeated and assigned to the short arm of the Y chromosome by in situ hybridization. Both sequences were detected in five out of six [corrected] 46,XX males and were reduced in copy number in one out of two 46,XY gonadal dysgenesis patients tested. The findings suggest close proximity of these Y-specific moderately repeated DNA sequences to a testis determining locus.

MeSH Terms
Cell Fractionation/methods Chromosome Mapping Flow Cytometry Gonadal Dysgenesis Humans Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid Sex Determination Analysis X Chromosome Y Chromosome
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Müller U
Lalande M
Donlon T
Latt S A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.3.1325
Published
1986-02-11
Pages
1325-40
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339507
Subset
IM
Grants
NIGMS NIH HHS · GM33579 · United States
NICHD NIH HHS · HD18658 · United States
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1093/nar/14.3.1325 PMC Full Text View journal details
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