Home LiteratureArticle Details
PMID: 3927298 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular cloning of Lyt-2, a membrane glycoprotein marking a subset of mouse T lymphocytes: molecular homology to its human counterpart, Leu-2/T8, and to immunoglobulin variable regions.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 15 ·1985-08-00 ·Pages 5126-30

Nakauchi H, Nolan GP, Hsu C, Huang HS, Kavathas P, Herzenberg LA

Abstract

The sequence of Lyt-2 cDNA shows that it is a new member of the immunoglobulin super gene family. Analysis of the predicted amino acid sequence indicates that the Lyt-2 polypeptide is synthesized with a 27-amino acid leader, and that the mature protein has an immunoglobulin variable region (Ig V)-related sequence of approximately 100 amino acids, an extracellular spacer of 43, a transmembrane region of 38, and an intracytoplasmic region of 27 amino acids. Lyt-2 and its human analogue Leu-2 are 56% homologous; analysis indicates that the Ig V-related domains of the two molecules have evolved away from each other faster than the carboxyl-terminal half of the proteins.

MeSH Terms
Amino Acid Sequence Animals Antigens, Differentiation, T-Lymphocyte Antigens, Ly/genetics Antigens, Surface/genetics Base Sequence Cloning, Molecular DNA/genetics Glycoproteins/genetics Humans Immunoglobulin Variable Region/genetics Membrane Proteins/genetics Mice Protein Conformation T-Lymphocytes/immunology
Chemicals
Antigens, Differentiation, T-Lymphocyte Antigens, Ly Antigens, Surface Glycoproteins Immunoglobulin Variable Region Membrane Proteins DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Nakauchi H
Nolan G P
Hsu C
Huang H S
Kavathas P
Herzenberg L A
References (38)
38 references, click to expand
  1. MRC OX-2 antigen: a lymphoid/neuronal membrane glycoprotein with a structure like a single immunoglobulin light chain.
    EMBO J. 1985 Jan;4(1):113-8 PMID: 2862025
  2. Ly antigens associated with T cell recognition and effector function.
    Immunol Rev. 1984 Dec;82:47-77 PMID: 6442265
  3. The three-dimensional structure of the fab' fragment of a human myeloma immunoglobulin at 2.0-angstrom resolution.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3440-4 PMID: 4215080
  4. Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen.
    J Exp Med. 1975 Jun 1;141(6):1376-89 PMID: 1092798
  5. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  6. Lymphocytes as models for the study of mammalian cellular differentiation.
    Immunol Rev. 1977 Jan;33:105-24 PMID: 300350
  7. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  8. Cytotoxic T cells: Lyt phenotype and blocking of killing activity by Lyt antisera.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1977-81 PMID: 88050
  9. Transformation of mammalian cells with genes from procaryotes and eucaryotes.
    Cell. 1979 Apr;16(4):777-85 PMID: 222468
  10. Mouse alloantibodies capable of blocking cytotoxic T-cell function. I. Relationship between the antigen reactive with blocking antibodies and the Lyt-2 locus.
    J Exp Med. 1979 Sep 19;150(3):432-44 PMID: 113478
  11. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  12. The differentiation and function of human T lymphocytes.
    Cell. 1980 Apr;19(4):821-7 PMID: 6991122
  13. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens.
    Immunol Rev. 1979;47:63-90 PMID: 398327
  14. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  15. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man.
    J Exp Med. 1981 Feb 1;153(2):310-23 PMID: 6165796
  16. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):544-8 PMID: 6787593
  17. Lyt-2-/Lyt 3- variants of a cloned cytolytic T cell line lack an antigen receptor functional in cytolysis.
    J Exp Med. 1981 Mar 1;153(3):595-604 PMID: 6788878
  18. Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity.
    J Exp Med. 1981 Jun 1;153(6):1503-16 PMID: 6166718
  19. Codon preference and its use in identifying protein coding regions in long DNA sequences.
    Nucleic Acids Res. 1982 Jan 11;10(1):141-56 PMID: 7063399
  20. Selective inhibition of human T cell cytotoxicity at levels of target recognition or initiation of lysis by monoclonal OKT3 and Leu-2a antibodies.
    J Exp Med. 1982 May 1;155(5):1579-84 PMID: 6978378
  21. Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin.
    Science. 1982 May 14;216(4547):696-703 PMID: 6177036
  22. An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences.
    Nucleic Acids Res. 1982 May 11;10(9):2951-61 PMID: 7099970
  23. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.
    Nucleic Acids Res. 1982 Aug 11;10(15):4731-51 PMID: 7133997
  24. Stable transformation of mouse L cells for human membrane T-cell differentiation antigens, HLA and beta 2-microglobulin: selection by fluorescence-activated cell sorting.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):524-8 PMID: 6188154
  25. Amplification of a gene coding for human T-cell differentiation antigen.
    Nature. 1983 Nov 24-30;306(5941):385-7 PMID: 6417545
  26. The immunoglobulin superfamily takes shape.
    Nature. 1984 Mar 1-7;308(5954):12-3 PMID: 6700707
  27. Isolation of cDNA clones encoding T cell-specific membrane-associated proteins.
    Nature. 1984 Mar 8-14;308(5955):149-53 PMID: 6199676
  28. The mouse Lyt-2/3 antigen complex--II. Structural analysis of the subunits.
    Mol Immunol. 1984 Apr;21(4):337-41 PMID: 6727877
  29. Somatic recombination in a murine T-cell receptor gene.
    Nature. 1984 May 24-30;309(5966):322-6 PMID: 6328306
  30. Complete primary structure of a heterodimeric T-cell receptor deduced from cDNA sequences.
    Nature. 1984 Jun 28-Jul 4;309(5971):757-62 PMID: 6330561
  31. Comparison of target antigens of monoclonal reagents OKT5, OKT8, and Leu2A, which inhibit effector function of human cytotoxic T lymphocytes.
    Hybridoma. 1983;2(2):187-99 PMID: 6205976
  32. A third type of murine T-cell receptor gene.
    Nature. 1984 Nov 1-7;312(5989):31-5 PMID: 6548551
  33. A third rearranged and expressed gene in a clone of cytotoxic T lymphocytes.
    Nature. 1984 Nov 1-7;312(5989):36-40 PMID: 6208487
  34. Isolation of the gene encoding the human T-lymphocyte differentiation antigen Leu-2 (T8) by gene transfer and cDNA subtraction.
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):7688-92 PMID: 6440142
  35. The isolation and sequence of the gene encoding T8: a molecule defining functional classes of T lymphocytes.
    Cell. 1985 Feb;40(2):237-46 PMID: 3871356
  36. Gene for the human T cell differentiation antigen Leu-2/T8 is closely linked to the kappa light chain locus on chromosome 2.
    J Exp Med. 1985 Feb 1;161(2):429-34 PMID: 3919142
  37. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  38. Genetic correlation of a mouse light chain variable region marker with a thymocyte surface antigen.
    J Exp Med. 1974 Nov 1;140(5):1432-7 PMID: 4547628
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.15.5126
Published
1985-08-00
Pages
5126-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390512
Subset
IM
Grants
NCI NIH HHS · CA-04681 · United States
NIGMS NIH HHS · GM-17367 · United States
NIGMS NIH HHS · GM-28428 · United States
Databases
GENBANK
M12825, M16980, M16981
External Links
PubMed source DOI: 10.1073/pnas.82.15.5126 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2