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PMID: 6788878 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Lyt-2-/Lyt 3- variants of a cloned cytolytic T cell line lack an antigen receptor functional in cytolysis.

The Journal of experimental medicine ·Vol. 153 ·No. 3 ·1981-03-01 ·Pages 595-604

Dialynas DP, Loken MR, Glasebrook AL, Fitch FW

Abstract

To investigate the role of Lyt-2 and Thy-1 in cytolysis, we have generated, by ethyl methanesulfonate mutagenesis and selection, variants of the cloned cytolytic T lymphocyte line L3 that specifically lack either Lyt-2 or Thy-1. An analysis of these variants indicates that neither Lyt-2 nor Lyt-3 is responsible for the lethal hit, but suggests that Lyt-2 and/or Lyt-3 are required for an antigen receptor functional in cytolysis. The data also suggest that the expression of Lyt-3 on the cell surface is not independent of the expression of Lyt-2. Finally the data indicate the Thy-1 plays no role in cytolysis.

MeSH Terms
Animals Antibodies Antibodies, Monoclonal Antigens, Surface Binding, Competitive Cells, Cultured Clone Cells/immunology Concanavalin A/pharmacology Cytotoxicity, Immunologic Ethyl Methanesulfonate/pharmacology Female Genetic Variation Isoantigens Mice Mice, Inbred C57BL Mice, Inbred DBA Phenotype Rats Rats, Inbred Lew Receptors, Antigen, T-Cell/immunology
Chemicals
Antibodies Antibodies, Monoclonal Antigens, Surface Isoantigens Receptors, Antigen, T-Cell Concanavalin A Ethyl Methanesulfonate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dialynas D P
Loken M R
Glasebrook A L
Fitch F W
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22 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.153.3.595
Published
1981-03-01
Pages
595-604
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2186095
Subset
IM
Grants
NIAID NIH HHS · AI-14872 · United States
NIAID NIH HHS · AI-16558 · United States
PHPPO CDC HHS · PHS2-T32 · United States
External Links
PubMed source DOI: 10.1084/jem.153.3.595 PMC Full Text View journal details
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