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PMID: 6188154 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Stable transformation of mouse L cells for human membrane T-cell differentiation antigens, HLA and beta 2-microglobulin: selection by fluorescence-activated cell sorting.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 2 ·1983-01-00 ·Pages 524-8

Kavathas P, Herzenberg LA

Abstract

We isolated stable transformants of mouse L cells expressing human cell surface differentiation antigens by using immunofluorescence with monoclonal antibodies and selection with a fluorescence-activated cell sorter (FACS). Mouse L cells (TK-) were cotransformed with human cellular DNA and the herpes simplex virus thymidine kinase (TK) gene. TK+ transformants were first selected. The TK+ populations were stained with various fluorescent antibodies to membrane antigens, and positive cells were sorted and cloned by using a FACS. Transformants for HLA class I antigens, for beta 2-microglobulin, and for the T-cell differentiation antigens Leu-1 and Leu-2 were isolated. The frequency of antigen transformants among the TK+ transformants was about 0.5 X 10(-3). The sizes of the HLA, Leu-1, and Leu-2 molecules expressed by the transformants were the same as those of the proteins present on DNA-donor cells.

MeSH Terms
Animals Antigens, Differentiation, T-Lymphocyte Antigens, Surface/immunology Beta-Globulins/immunology Cell Line Cell Transformation, Neoplastic Flow Cytometry Genes HLA Antigens/immunology Humans L Cells/immunology Leukemia, Lymphoid/immunology Mice Simplexvirus/enzymology,genetics T-Lymphocytes/immunology Thymidine Kinase/genetics beta 2-Microglobulin/immunology
Chemicals
Antigens, Differentiation, T-Lymphocyte Antigens, Surface Beta-Globulins HLA Antigens beta 2-Microglobulin Thymidine Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kavathas P
Herzenberg L A
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.2.524
Published
1983-01-00
Pages
524-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393411
Subset
IM
Grants
NCI NIH HHS · CA 04681 · United States
NIGMS NIH HHS · GM 17367 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.2.524 PMC Full Text View journal details
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