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PMID: 381202 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of protective cell surface proteins in ribosomal fractions from Salmonella typhimurium.

Infection and immunity ·Vol. 24 ·No. 3 ·1979-06-00 ·Pages 808-16

Misfeldt ML, Johnson W

Abstract

Cell surface antigen preparations from Salmonella typhimurium SR-11 prepared by either trichloroacetic acid extraction or boiling in sodium dodecyl sulfate were able to protect C3H/HeJ, C3H/HeDub, and A/J mice. Some of the proteins found in these preparations were shown to exist in the protective ribosomal fraction isolated from S. typhimurium SR-11. Passage of ribosomes isolated from S. typhimurium SR-11 and 6707 through a Sepharose 2B column removed the protective immunogen from 6707 ribosomes but did not completely remove it from SR-11 ribosomes. Immunity to salmonella infection in C3H/HeJ mice induced by ribosomal vaccines may be dependent on the presence of cell surface proteins in the ribosomal fraction.

MeSH Terms
Animals Antigens, Bacterial/immunology Antigens, Surface/immunology Bacterial Proteins/immunology Cell Wall/immunology Membrane Proteins/immunology Mice Mice, Inbred Strains Ribosomes/analysis,immunology Salmonella Infections, Animal/immunology Salmonella typhimurium/immunology,ultrastructure
Chemicals
Antigens, Bacterial Antigens, Surface Bacterial Proteins Membrane Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Misfeldt M L
Johnson W
References (38)
38 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.24.3.808-816.1979
Published
1979-06-00
Pages
808-16
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC414379
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.24.3.808-816.1979 PMC Full Text View journal details
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