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PMID: 4576683 Published · ppublish English Journal Article

Immunity to Salmonella typhimurium infection: characterization of antigens in active protection by polyacrylamide gel electrophoresis.

Infection and immunity ·Vol. 7 ·No. 3 ·1973-03-00 ·Pages 507-11

Houchens DP, Wright GL

Abstract

Immunity to challenge with Salmonella typhimurium (strain SR-11) was studied in NIH/NMRI Swiss mice. Viable organisms induced the best protection, but ribosomes were the best immunogen of the subcellular fractions. Antigens with lower concentrations of protein did not induce protection as well as antigens which had higher concentrations of protein. Acrylamide gel electrophoresis of the SR-11 subcellular components, fractionation, and differential staining of the gel columns indicated that the immunogen is a high-molecular-weight glycoprotein or mucopolysaccharide.

MeSH Terms
Animals Antigens, Bacterial/isolation & purification Electrophoresis, Polyacrylamide Gel Glycoproteins Immunization Mice Polysaccharides, Bacterial Ribosomes/immunology Salmonella Infections/immunology Salmonella typhimurium/immunology,pathogenicity
Chemicals
Antigens, Bacterial Glycoproteins Polysaccharides, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Houchens D P
Wright G L
References (19)
19 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.7.3.507-511.1973
Published
1973-03-00
Pages
507-11
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC422711
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.7.3.507-511.1973 PMC Full Text View journal details
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