Relationship of the structure of bacterial lipopolysaccharides to its function in mitogenesis and adjuvanticity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 7 ·1973-07-00 ·Pages 2129-33

Chiller JM, Skidmore BJ, Morrison DC, Weigle WO

Abstract

The ability of bacterial lipopolysaccharides to induce lymphocyte mitogenesis and to act as an adjuvant of antibody formation was attributable to the lipid-A region of the molecule. Measured by induction of DNA synthesis, lipid A was mitogenic for bone marrow-derived lymphocytes obtained from spleens of congenitally athymic mice but not for thymocytes obtained from thymuses of normal mice. Adjuvanticity was demonstrated by the ability of lipid A to convert a tolerogenic regimen of antigen into one eliciting an immune response and by its ability to markedly enhance the antibody response to a weak antigen.

MeSH Terms

Adjuvants, Immunologic Animals Antibody Formation/drug effects B-Lymphocytes/drug effects Escherichia coli/analysis Immune Tolerance/drug effects Immunization Lipopolysaccharides/isolation & purification,pharmacology Lymphocyte Activation Mice Mice, Inbred A Mitogens/pharmacology Polysaccharides, Bacterial/isolation & purification,pharmacology Salmonella/analysis Serum Albumin, Bovine Structure-Activity Relationship T-Lymphocytes/drug effects

Chemicals

Adjuvants, Immunologic Lipopolysaccharides Mitogens Polysaccharides, Bacterial Serum Albumin, Bovine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Chiller J M

Skidmore B J

Morrison D C

Weigle W O

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28 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.7.2129

Published

1973-07-00

Pages

2129-33

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433681

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.7.2129 PMC Full Text View journal details

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Relationship of the structure of bacterial lipopolysaccharides to its function in mitogenesis and adjuvanticity.

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