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PMID: 3600649 Published · ppublish English Journal Article

Specific growth inhibitory sequences in genomic DNA from quiescent human embryo fibroblasts.

Molecular and cellular biology ·Vol. 7 ·No. 5 ·1987-05-00 ·Pages 1894-9

Padmanabhan R, Howard TH, Howard BH

Abstract

We used HeLa cells as recipients in a gene transfer assay to characterize DNA sequences that negatively regulate mammalian cell growth. In this assay, genomic DNA from quiescent human embryo fibroblasts was more inhibitory for HeLa replication than was DNA from either Escherichia coli or HeLa cells. Surprisingly, growth inhibitory activity depended on the growth state of the cells from which genomic DNA was prepared; it was strongest in DNA prepared from serum-deprived, quiescent embryo fibroblasts. This latter observation implies a role for DNA modification(s) in regulating the activity of the inhibitory sequences detected in our assay. The level of the observed growth inhibitory activity was sometimes high, suggesting that the relevant sequences may be abundantly represented in the mammalian genome. We speculate that these findings may provide new insights into the molecular mechanisms involved in cellular quiescence and in vitro senescence.

MeSH Terms
Cell Differentiation Cell Division Cell Line Cell Survival DNA/physiology Fibroblasts/physiology Growth Inhibitors/genetics HeLa Cells/cytology Humans
Chemicals
Growth Inhibitors DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Padmanabhan R
Howard T H
Howard B H
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43 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.5.1894-1899.1987
Published
1987-05-00
Pages
1894-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365293
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.7.5.1894-1899.1987 PMC Full Text View journal details
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