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PMID: 3024165 Published · ppublish English Journal Article

Escherichia coli K-12 restricts DNA containing 5-methylcytosine.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 23 ·1986-12-00 ·Pages 9070-4

Raleigh EA, Wilson G

Abstract

We have observed that plasmids containing certain cloned modification methylase genes of type II restriction-modification systems cannot be transformed into many laboratory strains of Escherichia coli K-12. The investigation of this phenomenon, reported here, has revealed (i) DNA containing 5-methylcytosine is biologically restricted by these strains, while DNA containing 6-methyladenine is not; (ii) restriction is due to two genetically distinct systems that differ in their sequence specificities, which we have named mcrA and mcrB (for modified cytosine restriction). Since 5-methylcytosine containing DNA is widespread in nature, the Mcr systems probably have a broad biological role. Mcr restriction may seriously interfere with molecular cloning of 5-methylcytosine-containing foreign DNAs. The Mcr phenotypes of some commonly used strains of E. coli K-12 are reported.

MeSH Terms
5-Methylcytosine Bacteriophage lambda Base Sequence Cloning, Molecular/methods Cytosine/analogs & derivatives,metabolism DNA Restriction Enzymes/metabolism Escherichia coli/enzymology,genetics Genes, Bacterial Methyltransferases/genetics Phenotype Substrate Specificity
Chemicals
5-Methylcytosine Cytosine Methyltransferases DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Raleigh E A
Wilson G
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.23.9070
Published
1986-12-00
Pages
9070-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC387076
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.23.9070 PMC Full Text View journal details
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