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PMID: 1150745 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Reinitiation of DNA synthesis in senescent human fibroblasts upon fusion with cells of unlimited growth potential.

The Journal of cell biology ·Vol. 64 ·No. 3 ·1975-03-00 ·Pages 551-6

Norwood TH, Pendergrass WR, Martin GM

Abstract

Postreplicative, "senescent" human fibroblasts were fused to HeLa or to SV-40 transformed human fibroblasts with Sendai virus. DNA synthesis was reinitiated in senescent nuclei in a high proportion of the heterodikaryons. The [3H]thymidine labeling index of senescent fibroblast nuclei in heteropolykaryons was a function of the ratio of HeLa to senescent nuclei.

MeSH Terms
Cell Fusion Cell Nucleus/metabolism Cell Transformation, Neoplastic/metabolism DNA/biosynthesis DNA Replication Fibroblasts/metabolism HeLa Cells/metabolism Humans Hybrid Cells Thymidine/metabolism Time Factors
Chemicals
DNA Thymidine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Norwood T H
Pendergrass W R
Martin G M
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23 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.64.3.551
Published
1975-03-00
Pages
551-6
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109544
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.64.3.551 PMC Full Text View journal details
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