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PMID: 3600643 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure and expression of the chicken ferritin H-subunit gene.

Molecular and cellular biology ·Vol. 7 ·No. 5 ·1987-05-00 ·Pages 1751-8

Stevens PW, Dodgson JB, Engel JD

Abstract

Although the genomes of many species contain multiple copies of ferritin heavy (H)- and light (L)-chain sequences, the chicken genome contains only a single copy of the H-subunit gene. The primary transcription unit of this gene is 4.6 kilobase pairs and contains four exons which are posttranscriptionally spliced to generate a mature transcript of 869 nucleotides. Chicken and human ferritin H-subunit genomic loci are organized with similar exon-intron boundaries. They exhibit approximately 85% nucleotide identity in coding regions, which yield proteins 93% identical in amino acid sequence. We have identified a sequence of 22 highly conserved nucleotides in the 5' untranslated sequences of chicken, human, and tadpole ferritin H-subunit genes and propose that this conserved sequence may regulate iron-modulated translation of ferritin H-subunit mRNAs.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chickens Cloning, Molecular DNA/genetics Ferritins/genetics Gene Expression Regulation Genes Macromolecular Substances Multigene Family RNA, Messenger/genetics Sequence Homology, Nucleic Acid
Chemicals
Macromolecular Substances RNA, Messenger DNA Ferritins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stevens P W
Dodgson J B
Engel J D
References (43)
43 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.5.1751-1758.1987
Published
1987-05-00
Pages
1751-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365276
Subset
IM
Grants
NHLBI NIH HHS · HL24415 · United States
Databases
GENBANK
M16343
External Links
PubMed source DOI: 10.1128/mcb.7.5.1751-1758.1987 PMC Full Text View journal details
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