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PMID: 3889912 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation of recombinant cDNAs encoding chicken erythroid delta-aminolevulinate synthase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 11 ·1985-06-00 ·Pages 3702-6

Yamamoto M, Yew NS, Federspiel M, Dodgson JB, Hayashi N, Engel JD

Abstract

We report the isolation of cDNA clones encoding delta-aminolevulinate synthase (ALA synthase; EC 2.3.1.37), the first enzyme in the heme biosynthetic pathway in animal cells. The gene was isolated from a chicken erythroid cDNA library prepared in the bacteriophage lambda fusion/expression vector gt11, using rabbit antibody raised against the relatively abundant chicken liver enzyme. The chicken liver and red cell ALA synthase isozymes share substantial crossreactivity to the antibody, thereby allowing isolation of the erythroid-specific gene by using the heterologous antibody in immune screening of the red cell cDNA library. Preliminary analysis documenting the tissue specificity of transcription indicates that the enzyme is encoded by a highly homologous set of messages, which appear to differ in size in various avian tissues. From analysis using strand-specific RNA probes, it appears that the different ALA synthase mRNAs detected may be transcribed from a family of genes that are closely related in nucleotide sequence and are each regulated in a developmentally specific manner.

MeSH Terms
5-Aminolevulinate Synthetase/genetics Animals Chickens DNA/isolation & purification DNA, Recombinant/isolation & purification Erythrocytes/enzymology Gene Expression Regulation Immunosorbent Techniques Liver/enzymology RNA, Messenger/biosynthesis
Chemicals
DNA, Recombinant RNA, Messenger DNA 5-Aminolevulinate Synthetase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Yamamoto M
Yew N S
Federspiel M
Dodgson J B
Hayashi N
Engel J D
References (36)
36 references, click to expand
  1. Drug-induced porphyrin biosynthesis. V. Effect of protohemin on the transcriptional and post-transcriptional phases of -aminolevulinic acid synthetase induction.
    Biochem Pharmacol. 1972 Aug 1;21(15):2077-93 PMID: 4674939
  2. Mechanism of allylisopropylacetamide-induced increase of -aminolevulinate synthetase in liver mitochondria. V. Mechanism of regulation by hemin of the level of -aminolevulinate synthetase in rat liver mitochondria.
    Arch Biochem Biophys. 1972 Jan;148(1):10-21 PMID: 5058676
  3. Induction of delta-aminolevulinate synthetase in organ culture of chick embryo liver by allylisopropylacetamide and 3,5-dicarbethoxy-1,4-dihydrocollidine.
    J Biochem. 1974 May;75(5):1007 PMID: 4413844
  4. Two cell lines from lymphomas of Marek's disease.
    Biken J. 1974 Sep;17(3):105-16 PMID: 4616680
  5. Sequential induction of heme pathway enzymes during erythroid differentiation of mouse Friend leukemia virus-infected cells.
    J Exp Med. 1976 Feb 1;143(2):305-15 PMID: 1249519
  6. Synthesis of delta-aminolaevulinate synthase by isolated liver polyribosomes.
    Biochem J. 1976 Aug 15;158(2):391-400 PMID: 985436
  7. Analysis of chicken ribosomal RNA genes and construction of lambda hybrids containing gene fragments.
    Science. 1977 Apr 8;196(4286):195-7 PMID: 557836
  8. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  9. Isolation of the chicken beta-globin gene and a linked embryonic beta-like globin gene from a chicken DNA recombinant library.
    Cell. 1979 Aug;17(4):879-87 PMID: 158428
  10. Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927-31 PMID: 291909
  11. Translocation of delta-aminolevulinate synthase from the cytosol to the mitochondria and its regulation by hemin in the rat liver.
    J Biol Chem. 1980 Feb 25;255(4):1746-51 PMID: 7354050
  12. Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes.
    Cell. 1980 May;20(1):95-105 PMID: 6893015
  13. Electrophoretic transfer of proteins and nucleic acids from slab gels to diazobenzyloxymethyl cellulose or nitrocellulose sheets.
    Anal Biochem. 1980 Mar 1;102(2):459-71 PMID: 7425302
  14. Effects of hemin on the synthesis and intracellular translocation of delta-aminolevulinate synthase in the liver of rats treated with 3,5-dicarbethoxy-1,4-dihydrocollidine.
    J Biochem. 1980 Nov;88(5):1537-43 PMID: 7462193
  15. Haem control in experimental porphyria. The effect of haemin on the induction of delta-aminolaevulinate synthase in isolated chick-embryo liver cells.
    Biochem J. 1980 Jun 15;188(3):781-8 PMID: 7470035
  16. Evidence for erythroid and nonerythroid forms of delta-aminolevulinate synthetase.
    Arch Biochem Biophys. 1981 Feb;206(2):380-91 PMID: 7224646
  17. Regulation by heme of synthesis and intracellular translocation of delta-aminolevulinate synthase in the liver.
    Mol Cell Biochem. 1981 Jun 9;37(1):27-41 PMID: 6789140
  18. Regulation of synthesis and intracellular translocation of delta-aminolevulinate synthase by heme and its relation to the heme saturation of tryptophan pyrrolase in rat liver.
    Arch Biochem Biophys. 1981 Jul;209(2):451-9 PMID: 7294805
  19. Molecular cloning and characterization of Drosophila genes and their expression during embryonic development and in primary muscle cell cultures.
    Dev Biol. 1982 Apr;90(2):272-83 PMID: 6804285
  20. Determination of the molecular weight of detergent-solubilized enzymes by sedimentation equilibrium in an air-driven ultracentrifuge.
    Biochem Biophys Res Commun. 1982 Apr 14;105(3):895-901 PMID: 6124252
  21. Hormone-dependent terminal differentiation in vitro of chicken erythroleukemia cells transformed by ts mutants of avian erythroblastosis virus.
    Cell. 1982 Apr;28(4):907-19 PMID: 6954007
  22. Control of phage lambda development by stability and synthesis of cII protein: role of the viral cIII and host hflA, himA and himD genes.
    Cell. 1982 Dec;31(3 Pt 2):565-73 PMID: 6218885
  23. Efficient isolation of genes by using antibody probes.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8 PMID: 6219389
  24. Analysis of the adult chicken beta-globin gene. Nucleotide sequence of the locus, microheterogeneity at the 5'-end of beta-globin mRNA, and aberrant nuclear RNA species.
    J Biol Chem. 1983 Mar 25;258(6):3983-90 PMID: 6833240
  25. delta-Aminolevulinate synthase isozymes in the liver and erythroid cells of chicken.
    Biochem Biophys Res Commun. 1983 Jun 15;113(2):377-83 PMID: 6870861
  26. Translational inhibition by heme of the synthesis of hepatic delta-aminolevulinate synthase in a cell-free system.
    Biochem Biophys Res Commun. 1983 Aug 30;115(1):225-31 PMID: 6615529
  27. Identification of two distinct regulatory regions adjacent to the human beta-interferon gene.
    Cell. 1983 Oct;34(3):865-79 PMID: 6313211
  28. Yeast RNA polymerase II genes: isolation with antibody probes.
    Science. 1983 Nov 18;222(4625):778-82 PMID: 6356359
  29. India ink staining of proteins on nitrocellulose paper.
    Anal Biochem. 1983 Aug;133(1):157-62 PMID: 6195938
  30. Physical change in cytoplasmic messenger ribonucleoproteins in cells treated with inhibitors of mRNA transcription.
    Mol Cell Biol. 1984 Mar;4(3):415-23 PMID: 6717428
  31. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  32. The enzymatic synthesis of delta-aminolevulinic acid.
    J Biol Chem. 1958 Nov;233(5):1214-9 PMID: 13598764
  33. Different responses of the hepatic and erythropoietic delta-aminolevulinic acid synthetase of mice.
    Biochim Biophys Acta. 1967 Nov 28;148(2):585-7 PMID: 6075431
  34. Characterization and measurement of delta-aminolaevulinate synthetase in bone marrow cell mitochondria.
    Biochim Biophys Acta. 1968 Apr 24;159(1):27-37 PMID: 4297072
  35. Induction of -aminolevulinic acid synthetase in chick embryo liver cells in cluture.
    Proc Natl Acad Sci U S A. 1970 Oct;67(2):517-22 PMID: 4108609
  36. Studies of the perinatal differences in the activity of hepatic -aminolevulinic acid synthetase.
    Biochem Pharmacol. 1972 Jun 15;21(12):1735-44 PMID: 4649131
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.11.3702
Published
1985-06-00
Pages
3702-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397855
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.11.3702 PMC Full Text View journal details
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