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PMID: 3550436 Published · ppublish English Journal Article

Insulin induction of Xenopus laevis oocyte maturation is inhibited by monoclonal antibody against p21 ras proteins.

Molecular and cellular biology ·Vol. 7 ·No. 3 ·1987-03-00 ·Pages 1285-8

Deshpande AK, Kung HF

Abstract

Microinjection of transforming p21 ras protein induces maturation of Xenopus laevis oocytes, and the induction is blocked by coinjection of monoclonal antibody (Y13-259) against p21 ras proteins. Similar to other inducing agents, the effect of p21 ras protein is mediated via the appearance of maturation or meiosis-promoting factor activity. In addition, the neutralizing antibody markedly reduces oocyte maturation after insulin induction, whereas it fails to inhibit progesterone induction. Our results suggest that insulin induces maturation of oocytes via a different pathway than that of steroidal agents. The induction by insulin is ras dependent, and the action of ras may be directed at the steps before meiosis-promoting factor autocatalytic activation. These results suggest a role of p21 ras protein in the events associated with amphibian oocyte maturation.

MeSH Terms
Animals Antibodies, Monoclonal/administration & dosage Female Insulin/pharmacology Meiosis/drug effects Microinjections Oocytes/drug effects,growth & development Progesterone/pharmacology Proto-Oncogene Proteins/antagonists & inhibitors,pharmacology,physiology Xenopus laevis
Chemicals
Antibodies, Monoclonal Insulin Proto-Oncogene Proteins Progesterone
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Deshpande A K
Kung H F
References (51)
51 references, click to expand
  1. A bioassay for progesterone and gonadotropins based on the meiotic division of Xenopus oocytes in vitro.
    Gen Comp Endocrinol. 1971 Jun;16(3):599-605 PMID: 5087965
  2. THE R OLE OF THE GERMINAL VESICLE IN OOECYTE MATURATION IN ANURANS AS REVEALED BY THE REMOVAL AND TRANSPLANTATION OF NUCLEI.
    J Embryol Exp Morphol. 1964 Dec;12:851-73 PMID: 14251490
  3. Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
    J Exp Zool. 1971 Jun;177(2):129-45 PMID: 5106340
  4. Progesterone-induced maturational events in oocytes of Xenopus laevis. I. Continuous necessity for diffusible calcium and magnesium.
    Exp Cell Res. 1971 Sep;68(1):75-80 PMID: 5113962
  5. Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
    J Morphol. 1972 Feb;136(2):153-79 PMID: 4109871
  6. Studies on the appearance and nature of a maturation-inducing factor in the cytoplasm of amphibian oocytes exposed to progesterone.
    Dev Biol. 1974 Jun;38(2):394-400 PMID: 4545456
  7. Action of progesterone and related steroids on oocyte maturation in Xenopus laevis. An in vitro study.
    Cell Differ. 1972 Aug;1(3):179-89 PMID: 4670882
  8. Progesterone induced maturation in oocytes of Xenopus laevis. Appearance of a 'maturation promoting factor' in enucleated oocytes.
    Cell Differ. 1973 Oct;2(4):247-54 PMID: 4799790
  9. Hormone-induced maturation of Xenopus laevis oocytes: effects of different steroids and study of the properties of a progesterone receptor.
    Differentiation. 1974 Jun;2(3):129-35 PMID: 4374402
  10. Ovarian progesterone levels during in vitro oocyte maturation and ovulation in Xenopus laevis.
    Biol Reprod. 1975 Dec;13(5):561-7 PMID: 1203411
  11. A cytoplasmic factor promoting oocyte maturation: its extraction and preliminary characterization.
    Science. 1976 Mar 26;191(4233):1266-8 PMID: 1083070
  12. Defined nutrient medium for the in vitro maintenance of Xenopus laevis oocytes.
    In Vitro. 1976 Jun;12(6):418-27 PMID: 986365
  13. Initiation of meiotic maturation in Xenopus laevis oocytes by lanthanum.
    Nature. 1976 Jul 22;262(5566):289-90 PMID: 986558
  14. Primary action of steroid hormone at the surface of amphibian oocyte in the induction of germinal vesicle breakdown.
    Mol Cell Endocrinol. 1977 Nov;9(1):91-100 PMID: 201517
  15. Progesterone-induced meiotic reinitation in vitro in Xenopus laevis oocytes: a role for the displacement of membrane-bound calcium.
    Differentiation. 1977 Oct 20;9(1-2):67-76 PMID: 73488
  16. Meiotic maturation in Xenopus laevis oocytes initiated by insulin.
    Science. 1979 Sep 28;205(4413):1397-9 PMID: 472755
  17. Progesterone induces a rapid increase in [Ca2+]in of Xenopus laevis oocytes.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1534-6 PMID: 6929506
  18. The role of zinc and follicle cells in insulin-initiated meiotic maturation of Xenopus laevis oocytes.
    Science. 1980 Nov 21;210(4472):928-30 PMID: 7001631
  19. The p21 src genes of Harvey and Kirsten sarcoma viruses originate from divergent members of a family of normal vertebrate genes.
    Nature. 1981 Aug 6;292(5823):506-11 PMID: 6265801
  20. A study of the induction of cell division in amphibian oocytes by insulin.
    Dev Biol. 1981 Jul 30;85(2):309-16 PMID: 6266902
  21. In vitro induction of germinal vesicle breakdown in Xenopus laevis oocytes by melittin.
    Differentiation. 1982;21(2):127-32 PMID: 7084570
  22. Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family.
    J Virol. 1982 Jul;43(1):294-304 PMID: 6287003
  23. Mechanism of activation of a human oncogene.
    Nature. 1982 Nov 11;300(5888):143-9 PMID: 6290897
  24. A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene.
    Nature. 1982 Nov 11;300(5888):149-52 PMID: 7133135
  25. Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change.
    Nature. 1982 Dec 23;300(5894):762-5 PMID: 7177195
  26. Acquisition of transforming properties by alternative point mutations within c-bas/has human proto-oncogene.
    Nature. 1983 Jun 30;303(5920):775-9 PMID: 6866079
  27. Structure of the Ki-ras gene of the human lung carcinoma cell line Calu-1.
    Nature. 1983 Aug 11-17;304(5926):497-500 PMID: 6308465
  28. Activation of Ki-ras2 gene in human colon and lung carcinomas by two different point mutations.
    Nature. 1983 Aug 11-17;304(5926):507-13 PMID: 6308467
  29. Steroidal and peptidic control mechanisms in membrane of Xenopus laevis oocytes resuming meiotic division.
    J Steroid Biochem. 1983 Jul;19(1A):139-45 PMID: 6350717
  30. Structure and activation of the human N-ras gene.
    Cell. 1983 Sep;34(2):581-6 PMID: 6616621
  31. Presence of a Kirsten murine sarcoma virus ras oncogene in cells transformed by 3-methylcholanthrene.
    Mol Cell Biol. 1983 Dec;3(12):2298-301 PMID: 6197628
  32. Induction of mammary carcinomas in rats by nitroso-methylurea involves malignant activation of H-ras-1 locus by single point mutations.
    Nature. 1983 Dec 15-21;306(5944):658-61 PMID: 6318112
  33. Saccharomyces cerevisiae synthesizes proteins related to the p21 gene product of ras genes found in mammals.
    Mol Cell Biol. 1984 Jan;4(1):23-9 PMID: 6422257
  34. The Drosophila ras oncogenes: structure and nucleotide sequence.
    Cell. 1984 Jul;37(3):1027-33 PMID: 6430564
  35. Transformation of NIH 3T3 cells by microinjection of Ha-ras p21 protein.
    Nature. 1984 Aug 9-15;310(5977):508-11 PMID: 6611509
  36. Microinjection of the oncogene form of the human H-ras (T-24) protein results in rapid proliferation of quiescent cells.
    Cell. 1984 Aug;38(1):109-17 PMID: 6380758
  37. Expression of normal and transforming H-ras genes in Escherichia coli and purification of their encoded p21 proteins.
    Proc Natl Acad Sci U S A. 1984 Sep;81(17):5305-9 PMID: 6089191
  38. Developmental regulation of a Dictyostelium gene encoding a protein homologous to mammalian ras protein.
    Cell. 1984 Nov;39(1):141-8 PMID: 6091907
  39. Functional homology of mammalian and yeast RAS genes.
    Cell. 1985 Jan;40(1):19-26 PMID: 2981628
  40. Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells.
    Nature. 1985 Jan 17-23;313(5999):241-3 PMID: 3918269
  41. In vitro induction of Xenopus oocyte maturation by 4-pregnene-17 alpha, 20 beta-diol-3-one.
    Gen Comp Endocrinol. 1985 Jan;57(1):130-4 PMID: 3882519
  42. Mammalian and yeast ras gene products: biological function in their heterologous systems.
    Science. 1985 Apr 12;228(4696):179-84 PMID: 3883495
  43. Regulation of a ras-related protein during development of Dictyostelium discoideum.
    Mol Cell Biol. 1985 Jan;5(1):33-9 PMID: 3920511
  44. ras proteins can induce meiosis in Xenopus oocytes.
    Cell. 1985 Dec;43(3 Pt 2):615-21 PMID: 2416466
  45. Reversal of transformed phenotype by monoclonal antibodies against Ha-ras p21 proteins.
    Exp Cell Res. 1986 Feb;162(2):363-71 PMID: 3510879
  46. Requirement for c-ras proteins during viral oncogene transformation.
    Nature. 1986 Apr 10-16;320(6062):540-3 PMID: 2938016
  47. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture.
    Proc Natl Acad Sci U S A. 1986 Apr;83(8):2496-500 PMID: 3458212
  48. Antibodies to the ras gene product inhibit adenylate cyclase and accelerate progesterone-induced cell division in Xenopus laevis oocytes.
    Mol Cell Biol. 1986 Feb;6(2):719-22 PMID: 3537692
  49. A transforming ras gene can provide an essential function ordinarily supplied by an endogenous ras gene.
    Mol Cell Biol. 1986 May;6(5):1843-6 PMID: 2431287
  50. Induction of ovulation in vitro in Rana pipiens with steroids.
    Gen Comp Endocrinol. 1961 Apr;1:20-3 PMID: 13786809
  51. The interaction of steroids with Rana pipiens Oocytes in the induction of maturation.
    Dev Biol. 1971 Jun;25(2):232-47 PMID: 5562852
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.3.1285-1288.1987
Published
1987-03-00
Pages
1285-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365205
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.7.3.1285-1288.1987 PMC Full Text View journal details
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