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PMID: 986365 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Defined nutrient medium for the in vitro maintenance of Xenopus laevis oocytes.

In vitro ·Vol. 12 ·No. 6 ·1976-06-00 ·Pages 418-27

Eppig JJ, Dumont JN

Abstract

A procedure is described for the isolation and culture of large numbers of follicle cell-free Xenopus laevis oocytes in all stages of development. The isolation procedure involves the incubation of pieces of ovary in a calcium-free solution OR2 containing 0.2% collagenase. A defined nutrient medium for the maintenace of the oocytes in vitro is presented. It is shown that this medium, referred to as DNOM, can maintain certain morpological and functional characteristics of oocytes for periods up to 3 weeks.

MeSH Terms

Animals Cell Membrane/ultrastructure Cells, Cultured Chromosomes/ultrastructure Culture Media Endocytosis Female Oocytes/drug effects,metabolism,ultrastructure Ovum/ultrastructure Progesterone/pharmacology Protein Biosynthesis RNA/biosynthesis Vitellogenins/metabolism Xenopus

Chemicals

Culture Media Vitellogenins Progesterone RNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Eppig J J

Dumont J N

References (15)

15 references, click to expand

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Article Info

Journal

In vitro

Abbr.

In Vitro

ISSN

0073-5655

DOI

10.1007/BF02806021

Published

1976-06-00

Pages

418-27

Language

English

Region

United States

NLM ID

0063733

Subset

External Links

PubMed source DOI: 10.1007/BF02806021

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Defined nutrient medium for the in vitro maintenance of Xenopus laevis oocytes.

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