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PMID: 3519212 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Early replicative intermediates of Escherichia coli chromosome isolated from a membrane complex.

The EMBO journal ·Vol. 5 ·No. 4 ·1986-04-00 ·Pages 787-91

Yoshimoto M, Kambe-Honjoh H, Nagai K, Tamura G

Abstract

The replication origin region of the Escherichia coli chromosome was isolated from an outer membrane fraction. The chromosome was further purified by centrifugation in a cesium chloride gradient. Early replicative intermediates were enriched in the preparation when cytosine-1-beta-arabinofuranoside was added to the culture at the time of initiation of chromosome replication. DNA fragments with an eye structure having two branches of less than 400-500 bp in length were associated with components that were removed by phenol treatment. We conclude that the replication fork usually proceeds counter-clockwise toward the unc operon in the earliest period of replication.

MeSH Terms
Base Composition Cell Membrane/physiology Chromosomes, Bacterial/physiology,ultrastructure Cytarabine/pharmacology DNA Replication Escherichia coli/drug effects,genetics Microscopy, Electron
Chemicals
Cytarabine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yoshimoto M
Kambe-Honjoh H
Nagai K
Tamura G
References (37)
37 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-04-00
Pages
787-91
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1166859
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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