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PMID: 368576 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Restriction enzyme cleavage of DNA resulting from gently lysed Escherichia coli.

Molecular & general genetics : MGG ·Vol. 166 ·No. 3 ·1978-11-09 ·Pages 245-9

Valenzuela MS, Inman RB

Abstract

When E. coli or lambda infected E. coli are gently lysed the DNA is released as a very fast sedimenting species that is presumably bound to membrane material. If this complex is now subjected to restriction enzyme cleavage, only a minor fraction of the fast sedimenting DNA remains and this is found, after purification, to be enriched for branched molecules.

MeSH Terms
Cell Membrane/metabolism Coliphages/genetics DNA Replication DNA Restriction Enzymes/metabolism DNA, Bacterial/isolation & purification Escherichia coli/genetics
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Valenzuela M S
Inman R B
References (10)
10 references, click to expand
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    Biochem Biophys Res Commun. 1975 Aug 18;65(4):1221-8 PMID: 1052423
  5. Lack of a unique termination site for the first round of bacteriophage lambda DNA replication.
    J Mol Biol. 1976 Apr 15;102(3):569-89 PMID: 775112
  6. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
  7. Structure of branch points in replicating DNA: presence of single-stranded connections in lambda DNA branch points.
    J Mol Biol. 1971 Mar 14;56(2):319-25 PMID: 4927949
  8. The role of the host cell membrane in the replication and morphogenesis of bacteriophages.
    Annu Rev Microbiol. 1973;27:261-82 PMID: 4584688
  9. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.
    J Bacteriol. 1975 Jan;121(1):354-62 PMID: 1090574
  10. Chromosome--Membrane association in Bacillus subtilis. III. Isolation and characterization of a DNA-protein complex carrying replication origin markers.
    J Mol Biol. 1977 Feb 25;110(2):219-53 PMID: 15128
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267615
Published
1978-11-09
Pages
245-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267615
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