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PMID: 1094470 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Effect of growth conditions on DNA-membrane attachment in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 6 ·1975-06-00 ·Pages 2446-50

Parker DL, Glaser DA

Abstract

DNA-membrane attachment in cultures of E. coli B/r is found to depend on the pattern of DNA synthesis, (a) When the number of replication points increases, the fraction of the DNA that is membrane bound also increases. The relationship between the amount of DNA-membrane attachment and the number of replication points is most consistent with attachment of all replication points and attachment of at least one other chromosomal site; it is inconsistent with attachment of the replication points only, the chromosomal origin only, or the chromosomal terminus only. (b) When repair DNA synthesis is stimulated by irradiation with ultraviolet light, the DNA-membrane attachment remains unchanged.

MeSH Terms
Cell Membrane/metabolism,radiation effects DNA Repair DNA Replication DNA, Bacterial/biosynthesis,metabolism Escherichia coli/growth & development,metabolism,radiation effects,ultrastructure Mathematics Thymine/metabolism Ultraviolet Rays
Chemicals
DNA, Bacterial Thymine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Parker D L
Glaser D A
References (23)
23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.6.2446
Published
1975-06-00
Pages
2446-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432776
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.6.2446 PMC Full Text View journal details
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