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PMID: 1104640 Published · ppublish English Journal Article

Deoxyribonucleic acid-envelope complexes from Escherichia coli. A complex-specific protein and its possible function for the stability of the complex.

The Journal of cell biology ·Vol. 67 ·No. 2PT.1 ·1975-11-00 ·Pages 444-60

Heidrich HG, Olsen WL

Abstract

The different Escherichia coli envelope fractions (cell wall, cytoplasmic membrane, and DNA-envelope complex fragments) were isolated by free-flow electrophoresis and analyzed by sodium dodecylsulfate-acrylamide gel electrophoresis. The DNA-envelope complex fragments possess a specific protein (mol wt 80,000-90,000). Upon treatment with trypsin, this protein disappears and the complex breaks down, thus releasing DNA, cell wall, and cytoplasmic membrane. Disaggregation of the complex can also be achieved by high salt concentrations. Lysozyme treatment dissolves the murein layer within the complex but does not disaggregate the complex. From these and other results on the stability of the DNA-envelope complex, conclusions can be drawn about the possible linkage within the described envelope particles.

MeSH Terms
Bacterial Proteins/analysis,physiology Cell Fractionation Cell Membrane/analysis,drug effects,enzymology,metabolism Cell Wall/analysis,drug effects,metabolism DNA, Bacterial/isolation & purification,metabolism Deoxyribonucleases/pharmacology Escherichia coli/ultrastructure Muramidase/pharmacology Polyethylene Glycols/pharmacology Sodium Chloride/pharmacology Sodium Dodecyl Sulfate/pharmacology Subcellular Fractions/analysis Succinate Dehydrogenase/metabolism Trypsin/pharmacology
Chemicals
Bacterial Proteins DNA, Bacterial Sodium Dodecyl Sulfate Polyethylene Glycols Sodium Chloride Succinate Dehydrogenase Deoxyribonucleases Muramidase Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Heidrich H G
Olsen W L
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38 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.67.2.444
Published
1975-11-00
Pages
444-60
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109604
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.67.2.444 PMC Full Text View journal details
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