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PMID: 4216023 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation of DNA-membrane complex in Bacillus subtilis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 12 ·1974-12-00 ·Pages 4787-91

Sueoka N, Hammers JM

Abstract

A DNA-membrane complex of Bacillus subtilis was separated from the bulk of the membrane by CsCl-sucrose double gradient centrifugation. The complex has a CsCl-sucrose buoyant density of 1.35-1.45 g/cm(3). Gel electrophoretic analyses show that the complex has about 5% of the whole membrane proteins and contains proteins unique to the complex as well as a small amount of membrane proteins. DNA in the complex is enriched for a genetic marker close to the origin (pur A16) and for one near the terminus (metB5). Artifactual formation of the complex during the lysate preparation was shown to be unlikely.

MeSH Terms
Bacillus subtilis/ultrastructure Bacterial Proteins/analysis Carbon Radioisotopes Cell Fractionation Cell Membrane/analysis Centrifugation, Density Gradient DNA, Bacterial/isolation & purification Electrophoresis, Polyacrylamide Gel Leucine/metabolism Thymine/metabolism Tritium
Chemicals
Bacterial Proteins Carbon Radioisotopes DNA, Bacterial Tritium Leucine Thymine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sueoka N
Hammers J M
References (26)
26 references, click to expand
  1. Association of the Bacillus subtilis chromosome with the cell membrane: resolution of free and bound deoxyribonucleic acid on renografin gradients.
    J Bacteriol. 1970 Nov;104(2):839-50 PMID: 4992373
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Density gradient system. I. Formation and fractionation of density gradients.
    Anal Biochem. 1968 Oct 24;25(1):271-82 PMID: 5704749
  4. Initiation by the DNA-dependent RNA polymerase.
    Proc Natl Acad Sci U S A. 1966 Sep;56(3):1026-33 PMID: 5339336
  5. Association between the chromosome and the cytoplasmic membrane in Bacillus subtilis.
    Biochem Biophys Res Commun. 1969 May 8;35(3):354-62 PMID: 4977972
  6. Chromosome-membrane association in Bacillus subtilis. I. DNA release from membrane fraction.
    Biochem Biophys Res Commun. 1971 Sep 17;44(6):1559-65 PMID: 5003692
  7. Membrane attachment of the replication origins of a multifork (dichotomous) chromosome in Bacillus subtilis.
    J Mol Biol. 1972 Aug 21;69(2):237-48 PMID: 4627160
  8. Evidence for stable attachment of DNA to membrane at the replication origin of Escherichia coli.
    Biochem Biophys Res Commun. 1970 Oct 9;41(1):157-62 PMID: 4918012
  9. Some unique properties of the deoxyribonucleic acid-bearing portion of the bacterial membrane.
    J Bacteriol. 1972 Oct;112(1):195-9 PMID: 4627921
  10. Properties of the growing point region in the bacterial chromosome.
    Biochim Biophys Acta. 1967 Dec 19;149(2):519-31 PMID: 4866437
  11. Gene expression during outgrowth of Bacillus subtilis spores. The relationship between gene order on the chromosome and temporal sequence of enzyme synthesis.
    J Mol Biol. 1971 Aug 28;60(1):31-44 PMID: 4999102
  12. Development of competence in the Bacillus subtilis transformation system.
    J Bacteriol. 1967 Sep;94(3):562-70 PMID: 4962301
  13. Studies with DNA-cellulose chromatography. I. DNA-binding proteins from Escherichia coli.
    Cold Spring Harb Symp Quant Biol. 1968;33:289-305 PMID: 4891970
  14. Topography of chromosome membrane junction in Bacillus subtilis.
    Nat New Biol. 1973 Aug 15;244(137):204-6 PMID: 4199319
  15. Sequential replication of the Bacillus subtilis chromosome. IV. Genetic mapping by density transfer experiment.
    J Mol Biol. 1967 Jul 28;27(2):349-68 PMID: 4964542
  16. Sequential replication of Bacillus subtilis chromosome. I. Comparison of marker frequencies in exponential and stationary growth phases.
    Proc Natl Acad Sci U S A. 1963 Apr;49:559-66 PMID: 14002700
  17. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.
    Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8 PMID: 16590310
  18. Isolation of an RNA polymearse-DNA complex by CsCl equilibrium centrifugation.
    Biochem Biophys Res Commun. 1971 Dec 3;45(5):1255-61 PMID: 4944354
  19. Properties of a membrane-attached form of the folded chromosome of Escherichia coli.
    J Mol Biol. 1974 Jan 5;82(1):91-105 PMID: 4594427
  20. Lac repressor-operator interaction. I. Equilibrium studies.
    J Mol Biol. 1970 Feb 28;48(1):67-83 PMID: 4915295
  21. Some features of the bacterial membrane studied with the aid of a new fractionation technique.
    Biochem J. 1971 Apr;122(2):197-207 PMID: 5000708
  22. Analysis of bacteriophage T7 early RNAs and proteins on slab gels.
    J Mol Biol. 1973 Sep 15;79(2):237-48 PMID: 4760132
  23. [ELECTRON MICROSCOPE STUDY OF THE LINKAGE BETWEEN NUCLEUS AND MESOSOME IN BACILLUS SUBTILIS].
    Ann Inst Pasteur (Paris). 1964 Sep;107:384-400 PMID: 14190396
  24. Deoxyribonucleic acid-envelope complexes isolated from Escherichia coli by free-flow electrophoresis: biochemical and electron microscope characterization.
    J Bacteriol. 1974 May;118(2):646-53 PMID: 4597456
  25. Adenosine triphosphate-dependent deoxyribonuclease in Bacillus subtilis.
    J Biol Chem. 1973 Nov 10;248(21):7336-44 PMID: 4270471
  26. Membrane attachment of the chromosome replication origin in Bacillus subtilis.
    Cold Spring Harb Symp Quant Biol. 1968;33:695-705 PMID: 4977758
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.12.4787
Published
1974-12-00
Pages
4787-91
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433982
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.12.4787 PMC Full Text View journal details
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