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PMID: 5000708 Published · ppublish English Journal Article

Some features of the bacterial membrane studied with the aid of a new fractionation technique.

The Biochemical journal ·Vol. 122 ·No. 2 ·1971-04-00 ·Pages 197-207

Daniels MJ

Abstract

Membranes of Bacillus megaterium and other bacteria were bound to crystals of cadmium lauroylsarcosinate and were resolved into nine lipoprotein fractions by elution with potassium chloride and sodium deoxycholate solutions. The fractions differed widely in protein/lipid ratio. Some major protein species were probably common to all the fractions, but the phospholipid composition showed some variation. Electron-microscopic examination after negative staining revealed that material in certain fractions was in the form of particles of diameter about 12nm, and other fractions consisted of amorphous aggregates. The composition of the membrane in terms of the nine fractions was influenced markedly by the conditions of culture of the bacteria. Radioactive-labelling experiments suggested that some fractions served as precursors of others, and one fraction appeared to contain the attachment points of the DNA.

MeSH Terms
Bacillus megaterium/analysis Bacillus subtilis/analysis Bacteria/analysis Bile Acids and Salts Cadmium Cell Membrane/analysis Centrifugation, Density Gradient Culture Media Electrophoresis, Disc Escherichia coli/analysis Fatty Acids Kinetics Lipoproteins/analysis Microscopy, Electron Potassium Chloride Protein Binding Protoplasts Sarcosine
Chemicals
Bile Acids and Salts Culture Media Fatty Acids Lipoproteins Cadmium Potassium Chloride Sarcosine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Daniels M J
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21 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1220197
Published
1971-04-00
Pages
197-207
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1176763
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1220197 PMC Full Text View journal details
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