Some unique properties of the deoxyribonucleic acid-bearing portion of the bacterial membrane.

Journal of bacteriology ·Vol. 112 ·No. 1 ·1972-10-00 ·Pages 195-9

Ballesta JP, Cundliffe E, Daniels MJ, Silverstein JL, Susskind MM, Schaechter M

Abstract

By using the M-band technique we have shown that portions of the membranes of Bacillus megaterium and Escherichia coli vary in their affinity for magnesium-Sarkosyl crystals and in phospholipid composition. The portion to which deoxyribonucleic acid is attached comprises as little as 4% of the total cell membrane, has a particularly high degree of affinity for magnesium-Sarkosyl crystals, and is rich in phosphatidylethanolamine. The M-band fractionation does not depend on the use of lysozyme.

MeSH Terms

Bacillus megaterium/analysis,metabolism Bacterial Proteins/analysis Carbon Isotopes Cardiolipins/analysis Cell Fractionation Cell Membrane/analysis,drug effects,metabolism Centrifugation, Density Gradient Chromatography DNA, Bacterial/analysis,metabolism Escherichia coli/analysis,metabolism Glycerol Magnesium/pharmacology Muramidase Phosphates Phosphatidylethanolamines/analysis Phospholipids/analysis Phosphorus Isotopes Protoplasts/drug effects Surface-Active Agents/metabolism Tritium

Chemicals

Bacterial Proteins Carbon Isotopes Cardiolipins DNA, Bacterial Phosphates Phosphatidylethanolamines Phospholipids Phosphorus Isotopes Surface-Active Agents Tritium Muramidase Magnesium Glycerol

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Ballesta J P

Cundliffe E

Daniels M J

Silverstein J L

Susskind M M

Schaechter M

References (10)

10 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.112.1.195-199.1972

Published

1972-10-00

Pages

195-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC251396

Subset

External Links

PubMed source DOI: 10.1128/jb.112.1.195-199.1972 PMC Full Text View journal details

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Some unique properties of the deoxyribonucleic acid-bearing portion of the bacterial membrane.

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