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PMID: 6344019 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The 245 base-pair oriC sequence of the E. coli chromosome directs bidirectional replication at an adjacent region.

Nucleic acids research ·Vol. 11 ·No. 9 ·1983-05-11 ·Pages 2617-26

Tabata S, Oka A, Sugimoto K, Takanami M, Yasuda S, Hirota Y

Abstract

The replication origin of the E. coli K-12 chromosome has been isolated as autonomously replicating molecules(oriC plasmid), and the DNA region essential for replicating function(oriC) has been localized to a sequence of 232-245 base-pairs(bp) by deletion analysis. In this report, the functional role of oriC was analysed by using an in vitro replication system and various OriC+ and OriC- plasmids previously constructed. The results obtained were summarized as follows: (1) The oriC sequence contained information enough to direct bidirectional replication. (2) The actual DNA replication began at a region near, but outside, oriC and progressed bidirectionally. (3) Initiation of DNA synthesis at the specific region required the dnaA-complementing fraction from cells harboring a dnaA-carrying plasmid.

MeSH Terms

Base Sequence DNA Replication DNA, Bacterial/biosynthesis,genetics Escherichia coli/genetics Genes, Bacterial Plasmids Time Factors

Chemicals

DNA, Bacterial

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Tabata S

Oka A

Sugimoto K

Takanami M

Yasuda S

Hirota Y

References (12)

12 references, click to expand

Isolation and partial characterization of a mutant of Escherichia coli deficient in DNA polymerase II.
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2090-4 PMID: 4559593
Novel F prime factors able to replicate in Escherichia coli Hfr strains.
Proc Natl Acad Sci U S A. 1976 Jan;73(1):198-202 PMID: 1108015
Cloning and mapping of the replication origin of Escherichia coli.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5458-62 PMID: 341158
Origin of replication, oriC, or the Escherichia coli chromosome on specialized transducing phages lambda asn.
Mol Gen Genet. 1978 Apr 17;160(3):287-95 PMID: 353492
Bacteriophage lambda carrying the Escherichia coli chromosomal region of the replication origin.
Proc Natl Acad Sci U S A. 1978 Oct;75(10):5099-103 PMID: 368808
Nucleotide sequence of Escherichia coli K-12 replication origin.
Proc Natl Acad Sci U S A. 1979 Feb;76(2):575-9 PMID: 370832
Replication origin of the Escherichia coli K-12 chromosome: the size and structure of the minimum DNA segment carrying the information for autonomous replication.
Mol Gen Genet. 1980 Apr;178(1):9-20 PMID: 6991883
Enzymatic replication of the origin of the Escherichia coli chromosome.
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7370-4 PMID: 6278471
Enzymatic replication of E. coli chromosomal origin is bidirectional.
Nature. 1982 Apr 15;296(5858):623-7 PMID: 7040980
Structure of replication origin of the Escherichia coli K-12 chromosome: the presence of spacer sequences in the ori region carrying information for autonomous replication.
Nucleic Acids Res. 1982 Jun 25;10(12):3745-54 PMID: 6287420
An in vitro method generating base substitutions in preselected regions of plasmid DNA: application to structural analysis of the replication origin of the Escherichia coli K-12 chromosome.
Gene. 1982 Jul-Aug;19(1):59-69 PMID: 6292050
Promoters in the E. coli replication origin.
Nature. 1981 Nov 26;294(5839):376-8 PMID: 7031477

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/11.9.2617

Published

1983-05-11

Pages

2617-26

Language

English

Region

England

NLM ID

0411011

PMCID

PMC325912

Subset

External Links

PubMed source DOI: 10.1093/nar/11.9.2617 PMC Full Text View journal details

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The 245 base-pair oriC sequence of the E. coli chromosome directs bidirectional replication at an adjacent region.

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