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PMID: 3485173 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Differences in antigen presentation to MHC class I-and class II-restricted influenza virus-specific cytolytic T lymphocyte clones.

The Journal of experimental medicine ·Vol. 163 ·No. 4 ·1986-04-01 ·Pages 903-21

Morrison LA, Lukacher AE, Braciale VL, Fan DP, Braciale TJ

Abstract

We have examined requirements for antigen presentation to a panel of MHC class I-and class II-restricted, influenza virus-specific CTL clones by controlling the form of virus presented on the target cell surface. Both H-2K/D- and I region-restricted CTL recognize target cells exposed to infectious virus, but only the I region-restricted clones efficiently lysed histocompatible target cells pulsed with inactivated virus preparations. The isolated influenza hemagglutinin (HA) polypeptide also could sensitize target cells for recognition by class II-restricted, HA-specific CTL, but not by class I-restricted, HA-specific CTL. Inhibition of nascent viral protein synthesis abrogated the ability of target cells to present viral antigen relevant for class I-restricted CTL recognition. Significantly, presentation for class II-restricted recognition was unaffected in target cells exposed to preparations of either inactivated or infectious virus. This differential sensitivity suggested that these H-2I region-restricted CTL recognized viral polypeptides derived from the exogenously introduced virions, rather than viral polypeptides newly synthesized in the infected cell. In support of this contention, treatment of the target cells with the lysosomotropic agent chloroquine abolished recognition of infected target cells by class II-restricted CTL without diminishing class I-restricted recognition of infected target cells. Furthermore, when the influenza HA gene was introduced into target cells without exogenous HA polypeptide, the target cells that expressed the newly synthesized protein product of the HA gene were recognized only by H-2K/D-restricted CTL. These observations suggest that important differences may exist in requirements for antigen presentation between H-2K/D and H-2I region-restricted CTL. These differences may reflect the nature of the antigenic epitopes recognized by these two CTL subsets.

MeSH Terms
Animals Chloroquine/pharmacology Clone Cells Dose-Response Relationship, Immunologic Female Hemagglutinins/immunology Major Histocompatibility Complex Mice Mice, Inbred Strains Orthomyxoviridae/immunology T-Lymphocytes, Cytotoxic/immunology
Chemicals
Hemagglutinins Chloroquine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Morrison L A
Lukacher A E
Braciale V L
Fan D P
Braciale T J
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.163.4.903
Published
1986-04-01
Pages
903-21
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188068
Subset
IM
Grants
NIAID NIH HHS · AI-15353 · United States
NIAID NIH HHS · AI-15608 · United States
NHLBI NIH HHS · HL-33391 · United States
External Links
PubMed source DOI: 10.1084/jem.163.4.903 PMC Full Text View journal details
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