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PMID: 84044 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Lymphocyte specificity to protein antigens. II. Fine specificity of T-cell activation with cytochrome c and derived peptides as antigenic probes.

The Journal of experimental medicine ·Vol. 149 ·No. 2 ·1979-02-01 ·Pages 436-47

Corradin G, Chiller JM

Abstract

Murine T-lymphocyte specificity was determined in a system of antigen driven in vitro T-cell proliferation using cytochrome c molecules from different species, their derived peptides and reconstituted hybrid proteins. It was observed that primed T cells could discriminate between peptide fragments which differed from each other at a single amino acid residue. These conclusions were substantiated by the pattern of cross-reactivity noted in the response of closely related cytochrome c proteins as well as when artificial hybrid molecules reconstituted by the covalent linkage of peptide fragments were analyzed. The pattern of specificity observed appeared to be haplotype (BDF1) dependent although similar conclusions about the fine specificity of T cells in the response to cytochrome c have been obtained in other strains but associated with different residues.

MeSH Terms
Amino Acid Sequence Animals Cattle Cytochrome c Group/immunology Epitopes H-2 Antigens Horses Immunologic Memory Lymph Nodes/immunology Lymphocyte Activation Mice Peptide Fragments/immunology Rabbits Species Specificity T-Lymphocytes/immunology
Chemicals
Cytochrome c Group Epitopes H-2 Antigens Peptide Fragments
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Corradin G
Chiller J M
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29 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.149.2.436
Published
1979-02-01
Pages
436-47
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2184807
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.149.2.436 PMC Full Text View journal details
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