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PMID: 6580632 Published · ppublish English Journal Article

Construction and characterization of an infectious vaccinia virus recombinant that expresses the influenza hemagglutinin gene and induces resistance to influenza virus infection in hamsters.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 23 ·1983-12-00 ·Pages 7155-9

Smith GL, Murphy BR, Moss B

Abstract

A DNA copy of the influenza virus hemagglutinin gene, derived from influenza virus A/Jap/305/57 (H2N2) was inserted into the genome of vaccinia virus under the control of an early vaccinia virus promoter. Tissue culture cells infected with the purified recombinant virus synthesized influenza hemagglutinin, which was glycosylated and transported to the cell surface where it could be cleaved with trypsin into HA1 and HA2 subunits. Rabbits and hamsters inoculated intradermally with recombinant virus produced circulating antibodies that inhibited hemagglutination by influenza virus. Furthermore, vaccinated hamsters achieved levels of antibody similar to those obtained upon primary infection with influenza virus and were protected against respiratory infection with the A/Jap/305/57 influenza virus.

MeSH Terms
Animals Antibody Formation Cricetinae DNA, Recombinant/metabolism DNA, Viral/genetics Genes, Viral HeLa Cells Hemagglutinins, Viral/genetics Humans Immunity, Innate Influenza A virus/genetics,immunology,pathogenicity Influenza, Human/immunology Mesocricetus Transfection Vaccinia virus/genetics,immunology Viral Plaque Assay
Chemicals
DNA, Recombinant DNA, Viral Hemagglutinins, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Smith G L
Murphy B R
Moss B
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.23.7155
Published
1983-12-00
Pages
7155-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390012
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.23.7155 PMC Full Text View journal details
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