Functional expression in primate cells of cloned DNA coding for the hemagglutinin surface glycoprotein of influenza virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 9 ·1981-09-00 ·Pages 5488-92

Sveda MM, Lai CJ

Abstract

We have used simian virus 40 (SV40) DNA as a vector for expression of functional activity of a cloned influenza viral DNA segment in primate cells. Cloned full-length DNA sequences coding for the hemagglutinin of influenza A virus (Udorn/72/[H3N2]) were inserted into the late region of a viable deletion mutant of SV40, and the hybrid DNA was propagated in the presence of an early SV40 mutant (tsA28) helper. Infection of primate cells with the hybrid virus produced a polypeptide similar in molecular size to the hemagglutinin of influenza virus, as shown by immunoprecipitation and gel electrophoresis. The polypeptide was glycosylated, as shown by incorporation of radioactive sugars. The putative hemagglutinin exhibited functional activity, as shown by agglutination of erythrocytes. In addition, an indirect immunofluorescence assay showed that the hemagglutinin polypeptide of the hybrid virus could be detected on the surface of infected cells.

MeSH Terms

Animals Chlorocebus aethiops Cloning, Molecular/methods DNA, Recombinant Glycoproteins/genetics Hemagglutination Hemagglutinins, Viral/genetics Influenza A virus/genetics,immunology Simian virus 40/genetics Structure-Activity Relationship

Chemicals

DNA, Recombinant Glycoproteins Hemagglutinins, Viral

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sveda M M

Lai C J

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27 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.78.9.5488

Published

1981-09-00

Pages

5488-92

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC348771

Subset

External Links

PubMed source DOI: 10.1073/pnas.78.9.5488 PMC Full Text View journal details

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Functional expression in primate cells of cloned DNA coding for the hemagglutinin surface glycoprotein of influenza virus.

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