Home LiteratureArticle Details
PMID: 6198429 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cytotoxic T lymphocyte recognition of the influenza hemagglutinin gene product expressed by DNA-mediated gene transfer.

The Journal of experimental medicine ·Vol. 159 ·No. 2 ·1984-02-01 ·Pages 341-54

Braciale TJ, Braciale VL, Henkel TJ, Sambrook J, Gething MJ

Abstract

We have used the technique of DNA-mediated gene transfer to examine cytotoxic T lymphocyte (CTL) recognition of the product of the cloned A/JAPAN/305/57 hemagglutinin (HA) gene in murine (L929) cells. Using both heterogeneous and homogeneous (clonal) populations of type A influenza-specific CTL, we have demonstrated that the HA molecule can serve as a target antigen for both the subtype-specific and the cross-reactive subpopulations of influenza-specific CTL. Our results also raise the possibility that other virus-specified polypeptides may serve as target molecules for cross-reactive CTL.

MeSH Terms
Animals Cell Line Clone Cells/immunology Cross Reactions Epitopes Hemagglutinins, Viral/genetics,immunology Influenza A virus/genetics,immunology Male Mice Mice, Inbred BALB C Mice, Inbred CBA Orthomyxoviridae Infections/genetics,immunology Protein Biosynthesis Recombination, Genetic T-Lymphocytes, Cytotoxic/classification,immunology
Chemicals
Epitopes Hemagglutinins, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Braciale T J
Braciale V L
Henkel T J
Sambrook J
Gething M J
References (30)
30 references, click to expand
  1. Cross-reactivity for different type A influenza viruses of a cloned T-killer cell line.
    Nature. 1980 Nov 13;288(5787):164-5 PMID: 6968871
  2. Immunologic recognition of influenza virus-infected cells. I. Generation of a virus-strain specific and a cross-reactive subpopulation of cytotoxic T cells in the response to type A influenza viruses of different subtypes.
    Cell Immunol. 1977 Oct;33(2):423-36 PMID: 303150
  3. Expression of influenza A virus internal antigens on the surface of infected P815 cells.
    J Immunol. 1981 May;126(5):1814-9 PMID: 7217668
  4. Heterogeneity and specificity of cloned lines of influenza-virus specific cytotoxic T lymphocytes.
    J Exp Med. 1981 Apr 1;153(4):910-23 PMID: 6166708
  5. Simultaneous expression of H-2-restricted and alloreactive recognition by a cloned line of influenza virus-specific cytotoxic T lymphocytes.
    J Exp Med. 1981 May 1;153(5):1371-6 PMID: 6166717
  6. Cell-surface expression of influenza haemagglutinin from a cloned DNA copy of the RNA gene.
    Nature. 1981 Oct 22;293(5834):620-5 PMID: 6270568
  7. An analysis of effector T cell generation and function in mice exposed to influenza A or Sendai viruses.
    Immunol Rev. 1981;58:5-24 PMID: 6273293
  8. Antigen-specific human T lymphocyte clones: induction, antigen specificity, and MHC restriction of influenza virus-immune clones.
    J Immunol. 1982 Jan;128(1):233-8 PMID: 6172480
  9. Surface expression of a nonstructural antigen on influenza A virus-infected cells.
    Infect Immun. 1981 Dec;34(3):1065-7 PMID: 7037639
  10. The interactionof antiody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies.
    Virology. 1982 Aug;121(1):157-67 PMID: 6180550
  11. Construction of influenza haemagglutinin genes that code for intracellular and secreted forms of the protein.
    Nature. 1982 Dec 16;300(5893):598-603 PMID: 7144911
  12. Influenza A specific cytotoxic T-cell clones that do not recognize viral glycoproteins.
    Nature. 1982 Dec 16;300(5893):655-7 PMID: 6183597
  13. Preparation of influenza virus subviral particles lacking the HA1 subunit of hemagglutinin: unmasking of cross-reactive HA2 determinants.
    Virology. 1983 Apr 15;126(1):106-16 PMID: 6189287
  14. Cytotoxic T cells to type A influenza virus; viral hemagglutinin induces A-strain specificity while infected cells confer cross-reactive cytotoxicity.
    Eur J Immunol. 1977 Sep;7(9):630-5 PMID: 303568
  15. Transfer of specific cytotoxic T lymphocytes protects mice inoculated with influenza virus.
    Nature. 1978 May 18;273(5659):238-9 PMID: 306072
  16. Antigen-specific suppressor T-cell activity in genetically restricted immune spleen cells.
    J Exp Med. 1978 Nov 1;148(5):1271-81 PMID: 102722
  17. Specificity of cytotoxicity T cells directed to influenza virus hemagglutinin.
    J Exp Med. 1979 Apr 1;149(4):856-69 PMID: 311817
  18. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373-6 PMID: 286319
  19. Role of T-cell function in recovery from murine influenza infection.
    Cell Immunol. 1979 Mar 15;43(2):341-51 PMID: 113108
  20. Cross-reactive cytotoxic T cells to serologically distinct vesicular stomatitis virus.
    J Immunol. 1980 May;124(5):2301-8 PMID: 6154097
  21. Recognition of viral glycoproteins by influenza A-specific cross-reactive cytolytic T lymphocytes.
    J Exp Med. 1980 Apr 1;151(4):945-58 PMID: 6154763
  22. Quantitation of influenza virus antigens on infected target cells and their recognition by cross-reactive cytotoxic T cells.
    J Exp Med. 1980 May 1;151(5):1014-25 PMID: 6966315
  23. Isolation of the chicken thymidine kinase gene by plasmid rescue.
    Nature. 1980 May 22;285(5762):207-10 PMID: 6246445
  24. Cellular immune responses of mice to influenza virus vaccines.
    J Immunol. 1980 Nov;125(5):2182-8 PMID: 7191866
  25. Generation of both cross-reactive and virus-specific T-cell populations after immunization with serologically distinct influenza A viruses.
    J Exp Med. 1977 Mar 1;145(3):557-68 PMID: 233901
  26. Cytotoxic T cells specific for influenza virus-infected target cells.
    Immunology. 1977 Feb;32(2):151-9 PMID: 300355
  27. Cytotoxic T cells kill influenza virus infected cells but do not distinguish between serologically distinct type A viruses.
    Nature. 1977 May 26;267(5609):354-6 PMID: 194164
  28. Immunologic recognition of influenza virus-infected cells. II. Expression of influenza A matrix protein on the infected cell surface and its role in recognition by cross-reactive cytotoxic T cells.
    J Exp Med. 1977 Sep 1;146(3):673-89 PMID: 302303
  29. Antibody to influenza virus matrix protein detects a common antigen on the surface of cells infected with type A influenza viruses.
    J Exp Med. 1977 Sep 1;146(3):690-7 PMID: 70496
  30. Cells mediating delayed-type hypersensitivity in the lungs of mice infected with an influenza A virus.
    Scand J Immunol. 1980;12(5):393-400 PMID: 7466327
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.159.2.341
Published
1984-02-01
Pages
341-54
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187223
Subset
IM
Grants
NIAID NIH HHS · AI-15353 · United States
NIAID NIH HHS · AI-15608 · United States
NIAID NIH HHS · AI-19630 · United States
External Links
PubMed source DOI: 10.1084/jem.159.2.341 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2