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PMID: 3467175 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Coding sequence and growth regulation of the human vimentin gene.

Molecular and cellular biology ·Vol. 6 ·No. 11 ·1986-11-00 ·Pages 3614-20

Ferrari S, Battini R, Kaczmarek L, Rittling S, Calabretta B, de Riel JK, Philiponis V, Wei JF, Baserga R

Abstract

We have established the complete coding sequence of the human vimentin gene. It had 91% homology to the coding sequence of the Syrian hamster vimentin gene (Quax et al., Cell 35:215-223, 1983) and partial homology to several other sequences coding for intermediate filament proteins. The most striking difference between the Syrian hamster and human vimentin genes was in the 3' untranslated region, which was considerably longer in the Syrian hamster. Using RNA blots and a human vimentin cDNA clone from an Okayama-Berg library, we have established that expression of the vimentin gene was growth regulated. The steady-state levels of cytoplasmic vimentin mRNA in 3T3 cells were increased by serum and platelet-derived growth factor, but not by epidermal growth factor, insulin, or platelet-poor plasma. The increase in expression of the vimentin gene that occurred when G0-phase cells were stimulated to proliferate was detected in six different cell types from four different species. The expression of the vimentin gene was also increased when HL60 cells were induced to differentiate by phorbol esters; it decreased when differentiation was induced by retinoic acid.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cells, Cultured Cloning, Molecular DNA/metabolism Genes/drug effects Growth Substances/pharmacology Humans Leukemia, Myeloid, Acute Mice Mice, Inbred BALB C Vimentin/genetics
Chemicals
Growth Substances Vimentin DNA
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Ferrari S
Battini R
Kaczmarek L
Rittling S
Calabretta B
de Riel J K
Philiponis V
Wei J F
Baserga R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.11.3614-3620.1986
Published
1986-11-00
Pages
3614-20
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367121
Subset
IM
Grants
NCI NIH HHS · CA-25898 · United States
NIGMS NIH HHS · GM-33694 · United States
Databases
GENBANK
M14144
External Links
PubMed source DOI: 10.1128/mcb.6.11.3614-3620.1986 PMC Full Text View journal details
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