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PMID: 6376521 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Differentiation of murine erythroleukemia cells results in the rapid repression of vimentin gene expression.

The Journal of cell biology ·Vol. 99 ·No. 1 Pt 1 ·1984-07-00 ·Pages 306-14

Ngai J, Capetanaki YG, Lazarides E

Abstract

We show that vimentin filaments are present in undifferentiated Friend murine erythroleukemia cells, but are lost progressively to undetectable levels by 96 h of dimethyl sulfoxide-mediated differentiation. The amount of newly synthesized cytoskeletal vimentin is decreased dramatically by 24 h of induction, and is paralleled by a rapid loss of vimentin mRNA (approximately 25-fold reduction at 96 h). Hence, disappearance of vimentin filaments in these cells appears to be regulated at the level of vimentin mRNA abundance. On the other hand, the levels of actin synthesis and actin mRNA remain essentially unchanged. The kinetics of vimentin mRNA reduction during dimethyl sulfoxide-mediated differentiation, and the levels of vimentin mRNA observed in the presence of hexamethylene-bisacetamide or hemin as inducers suggest that the cessation of vimentin expression precedes, but may be associated with commitment to terminal differentiation. Our results demonstrate the dynamic regulation of vimentin expression in mammalian erythropoiesis.

MeSH Terms
Acetamides/pharmacology Actins/genetics Animals Cell Differentiation Chickens Cytoskeleton/metabolism Dimethyl Sulfoxide/pharmacology Fluorescent Antibody Technique Gene Expression Regulation Globins/genetics Hemin/pharmacology Intermediate Filament Proteins/genetics Leukemia, Erythroblastic, Acute/pathology Mice Microscopy, Fluorescence RNA, Messenger/metabolism Rabbits Vimentin
Chemicals
Acetamides Actins Intermediate Filament Proteins RNA, Messenger Vimentin Hemin Globins hexamethylene bisacetamide Dimethyl Sulfoxide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ngai J
Capetanaki Y G
Lazarides E
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60 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.99.1.306
Published
1984-07-00
Pages
306-14
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2275651
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.99.1.306 PMC Full Text View journal details
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