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PMID: 3422482 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transforming growth factor beta increases mRNA for matrix proteins both in the presence and in the absence of changes in mRNA stability.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 4 ·1988-02-00 ·Pages 1105-8

Penttinen RP, Kobayashi S, Bornstein P

Abstract

Transforming growth factor-beta (TGF-beta) has been shown to stimulate synthesis of extracellular matrix proteins, both in animals and in cell culture. We found that mRNAs for alpha 1(I) collagen, fibronectin, and thrombospondin were markedly increased in TGF-beta-treated 3T3 (mouse) cells. For collagen and fibronectin this increase was 10-to 20-fold, as measured by quantitative blot hybridization analysis. A maximal value was reached at 16-24 hr, with a subsequent gradual decline. Concomitant treatment with cycloheximide prevented the stimulation observed with TGF-beta. Under conditions of confluent growth a clear increase in alpha 1(I) collagen mRNA stability was observed, whereas in subconfluent cells no change in mRNA half-life was found, despite an equally large increase in mRNA levels. We suggest that the mode of action of TGF-beta varies with the target cell and depends on the interplay of a number of complex cellular factors.

MeSH Terms
Animals Cell Line Collagen/biosynthesis Extracellular Matrix/drug effects,metabolism Fibronectins/biosynthesis Gene Expression Regulation/drug effects Glycoproteins/biosynthesis Mice Peptides/pharmacology RNA, Messenger/metabolism Stimulation, Chemical Thrombospondins Transforming Growth Factors
Chemicals
Fibronectins Glycoproteins Peptides RNA, Messenger Thrombospondins Transforming Growth Factors Collagen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Penttinen R P
Department of Biochemistry, University of Washington, Seattle 98195.
Kobayashi S
Bornstein P
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.4.1105
Published
1988-02-00
Pages
1105-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279714
Subset
IM
Grants
NIADDK NIH HHS · AM11248 · United States
NIDCR NIH HHS · DE02600 · United States
NHLBI NIH HHS · HL18645 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.4.1105 PMC Full Text View journal details
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