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PMID: 3457014 Published · ppublish English Journal Article

Type beta transforming growth factor in human platelets: release during platelet degranulation and action on vascular smooth muscle cells.

The Journal of cell biology ·Vol. 102 ·No. 4 ·1986-04-00 ·Pages 1217-23

Assoian RK, Sporn MB

Abstract

A specific radioimmunoassay for type beta transforming growth factor (TGF-beta) was developed and used to show that human platelets treated with thrombin release TGF-beta as a consequence of degranulation. The thrombin concentrations required to induce release of TGF-beta parallel those concentrations that release the alpha-granule marker, beta-thromboglobulin. Related studies showed that TGF-beta acts on early passage, explant cultures of bovine aortic smooth muscle cells by inhibiting the effect of mitogens on proliferation of subconfluent cell monolayers yet synergizing with mitogens to stimulate growth of the same cells when cultured in soft agar. The results show that primary cultures of bovine aortic smooth muscle cells and established normal rat kidney cells behave similarly with regard to TGF-beta action. Moreover, the data suggest that platelet-mediated proliferation of aortic smooth muscle cells in vivo may not result solely from the stimulatory effect of platelet-derived growth factor (PDGF), but rather from an interaction of platelet factors which has the intrinsic ability to limit as well as stimulate mitosis.

MeSH Terms
Animals Aorta/physiology Blood Platelets/metabolism,physiology Cattle Cell Division/drug effects Cell Line Cells, Cultured Growth Substances/blood Humans Kidney Kinetics Muscle, Smooth, Vascular/cytology,drug effects,physiology Peptides/blood Radioimmunoassay Rats Thrombin/physiology Transforming Growth Factors
Chemicals
Growth Substances Peptides Transforming Growth Factors Thrombin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Assoian R K
Sporn M B
References (46)
46 references, click to expand
  1. Platelet-derived growth factor is structurally related to the putative transforming protein p28sis of simian sarcoma virus.
    Nature. 1983 Jul 7-13;304(5921):35-9 PMID: 6306471
  2. Simian sarcoma virus onc gene, v-sis, is derived from the gene (or genes) encoding a platelet-derived growth factor.
    Science. 1983 Jul 15;221(4607):275-7 PMID: 6304883
  3. Cellular responsiveness to growth factors correlates with a cell's ability to express the transformed phenotype.
    Cell. 1983 Jul;33(3):931-8 PMID: 6307528
  4. Human plasma epidermal growth factor/beta-urogastrone is associated with blood platelets.
    J Clin Invest. 1983 Jul;72(1):249-59 PMID: 6603475
  5. Platelet-derived growth factor elicits cyclic AMP accumulation in Swiss 3T3 cells: role of prostaglandin production.
    Cell. 1983 Aug;34(1):265-72 PMID: 6192934
  6. Is epidermal growth factor present in human blood? Interference with the radioreceptor assay for epidermal growth factor.
    Biochem Biophys Res Commun. 1983 Aug 12;114(3):1036-41 PMID: 6311192
  7. Production of platelet-derived growth factor-like molecules and reduced expression of platelet-derived growth factor receptors accompany transformation by a wide spectrum of agents.
    Proc Natl Acad Sci U S A. 1984 Apr;81(8):2396-400 PMID: 6326125
  8. Cellular transformation by coordinated action of three peptide growth factors from human platelets.
    Nature. 1984 Jun 28-Jul 4;309(5971):804-6 PMID: 6330562
  9. Type beta transforming growth factor from feline sarcoma virus-transformed rat cells. Isolation and biological properties.
    J Biol Chem. 1984 Aug 10;259(15):9756-61 PMID: 6086646
  10. Characterization of a membrane receptor for transforming growth factor-beta in normal rat kidney fibroblasts.
    J Biol Chem. 1984 Sep 10;259(17):10995-1000 PMID: 6088525
  11. Developmentally regulated production of platelet-derived growth factor-like molecules.
    Nature. 1984 Oct 18-24;311(5987):669-71 PMID: 6090952
  12. Growth inhibitor from BSC-1 cells closely related to platelet type beta transforming growth factor.
    Science. 1984 Nov 9;226(4675):705-7 PMID: 6093254
  13. Type beta transforming growth factor: a bifunctional regulator of cellular growth.
    Proc Natl Acad Sci U S A. 1985 Jan;82(1):119-23 PMID: 3871521
  14. Purification and properties of porcine platelet-derived growth factor.
    EMBO J. 1984 Dec 1;3(12):2963-7 PMID: 6526008
  15. Further study of beta-TGFs released by virally transformed and non-transformed cells.
    Int J Cancer. 1985 Apr 15;35(4):553-8 PMID: 2985512
  16. Stimulation by insulin-like growth factors is required for cellular transformation by type beta transforming growth factor.
    J Biol Chem. 1985 Apr 25;260(8):4551-4 PMID: 2985553
  17. Type beta transforming growth factor/growth inhibitor stimulates entry of monolayer cultures of AKR-2B cells into S phase after a prolonged prereplicative interval.
    Proc Natl Acad Sci U S A. 1985 Jun;82(12):4147-51 PMID: 3858871
  18. Arterial smooth muscle cells in primary culture produce a platelet-derived growth factor-like protein.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4418-22 PMID: 2989820
  19. Biphasic effects of type beta transforming growth factor on epidermal growth factor receptors in NRK fibroblasts. Functional consequences for epidermal growth factor-stimulated mitosis.
    J Biol Chem. 1985 Aug 15;260(17):9613-7 PMID: 2991254
  20. A significant part of macrophage-derived growth factor consists of at least two forms of PDGF.
    Cell. 1985 Nov;43(1):277-86 PMID: 2416458
  21. The smooth muscle cell. II. Growth of smooth muscle in culture and formation of elastic fibers.
    J Cell Biol. 1971 Jul;50(1):172-86 PMID: 4327464
  22. Epidermal growth factor and a new derivative. Rapid isolation procedures and biological and chemical characterization.
    J Biol Chem. 1972 Dec 10;247(23):7609-11 PMID: 4636326
  23. A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1207-10 PMID: 4208546
  24. Intimal injury and regrowth in the rabbit aorta; medial smooth muscle cells as a source of neointima.
    Circ Res. 1975 Jan;36(1):58-70 PMID: 1116230
  25. The pathogenesis of atherosclerosis (first of two parts).
    N Engl J Med. 1976 Aug 12;295(7):369-77 PMID: 819830
  26. Radioimmunoassay of platelet factor 4 and beta-thromboglobulin: development and application to studies of platelet release in relation to fibrinopeptide A generation.
    Br J Haematol. 1978 May;39(1):129-46 PMID: 78721
  27. The platelet-derived growth factor.
    Cell. 1978 Jun;14(2):203-10 PMID: 352535
  28. Platelet alpha-granule proteins: studies on release and subcellular localization.
    Blood. 1979 Apr;53(4):604-18 PMID: 426909
  29. Platelet alpha granules contain a growth factor for fibroblasts.
    Blood. 1979 Jun;53(6):1043-52 PMID: 444648
  30. Platelet-derived growth factor: purification and partial characterization.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3722-6 PMID: 291037
  31. Transforming growth factors: isolation of polypeptides from virally and chemically transformed cells by acid/ethanol extraction.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3494-8 PMID: 6251462
  32. Endothelial regeneration. III. Time course of intimal changes after small defined injury to rat aortic endothelium.
    Lab Invest. 1981 Apr;44(4):301-8 PMID: 7206628
  33. Attachment of smooth muscle cells to collagen and their migration toward platelet-derived growth factor.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3669-72 PMID: 6943570
  34. New class of transforming growth factors potentiated by epidermal growth factor: isolation from non-neoplastic tissues.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5339-43 PMID: 6975480
  35. Platelet-derived growth factor in chemotactic for fibroblasts.
    J Cell Biol. 1982 Feb;92(2):584-8 PMID: 7061598
  36. Effect of platelet-derived and endothelial cell-derived growth factors on the low density lipoprotein receptor pathway in cultured human fibroblasts.
    J Biol Chem. 1982 May 25;257(10):5392-401 PMID: 6279599
  37. Platelet-derived growth factor. I. High yield purification and evidence for multiple forms.
    J Biol Chem. 1982 May 10;257(9):5154-60 PMID: 7068680
  38. Synergistic interaction of two classes of transforming growth factors from murine sarcoma cells.
    Cancer Res. 1982 Nov;42(11):4776-8 PMID: 6290046
  39. Serum contains a platelet-derived transforming growth factor.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5312-6 PMID: 6957865
  40. Polypeptide transforming growth factors isolated from bovine sources and used for wound healing in vivo.
    Science. 1983 Mar 18;219(4590):1329-31 PMID: 6572416
  41. Comparison of intra- and extracellular transforming growth factors from nontransformed and chemically transformed mouse embryo cells.
    Cancer Res. 1983 Apr;43(4):1581-6 PMID: 6299525
  42. Chemotactic activity of platelet alpha granule proteins for fibroblasts.
    J Cell Biol. 1983 Feb;96(2):382-5 PMID: 6187750
  43. Cultured endothelial cells produce a platelet-derived growth factor-like protein.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):1919-23 PMID: 6300879
  44. Transforming growth factors from neoplastic and nonneoplastic tissues.
    Fed Proc. 1983 Jun;42(9):2621-6 PMID: 6303865
  45. Transforming growth factor-beta in human platelets. Identification of a major storage site, purification, and characterization.
    J Biol Chem. 1983 Jun 10;258(11):7155-60 PMID: 6602130
  46. The molecular biology of platelet-derived growth factor.
    Cell. 1983 Jul;33(3):653-5 PMID: 6307524
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.102.4.1217
Published
1986-04-00
Pages
1217-23
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114151
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.102.4.1217 PMC Full Text View journal details
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