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PMID: 6957865 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Serum contains a platelet-derived transforming growth factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 17 ·1982-09-00 ·Pages 5312-6

Childs CB, Proper JA, Tucker RF, Moses HL

Abstract

High concentrations of fetal bovine serum induced colony formation in soft agar by anchorage-dependent, nontransformed mouse AKR-2B and rat NRK cells. The colony-stimulating activity in fetal bovine serum was precipitated by 45% saturated ammonium sulfate and migrated in molecular sieve chromatography as a single peak of activity in the 10,000-15,000 molecular weight range. The colony-stimulating activity was heat and acid stable and was destroyed by trypsin and dithiothreitol, indicating the activity is due to a polypeptide that requires disulfide bonds for biological activity. No competition for binding to the epidermal growth factor receptor was associated with the colony-stimulating activity. Isoelectric focusing revealed activity in the pI 4-5 range. The colony-stimulating activity in serum appeared to be of platelet origin because platelet-poor plasma and platelet-poor plasma-derived serum contained little activity, whereas acid/ethanol extracts of bovine and human platelets had potent colony-stimulating activity. Chromatography of platelet extracts on Bio-Gel P-60 revealed peaks of AKR-2B colony-stimulating activity in the 12,000 and 20,000 molecular weight ranges. The other biological and chemical properties of the platelet colony-stimulating activity were the same as those for the serum activity. The data indicate the presence in serum of a platelet-derived growth factor(s) with properties similar to those of the transforming growth factors.

MeSH Terms
Animals Biological Assay Blood Platelets/analysis Cattle Cell Division/drug effects Cell Line Fetal Blood/analysis Fetus Growth Substances/isolation & purification,pharmacology Humans Peptides/isolation & purification,pharmacology Platelet-Derived Growth Factor
Chemicals
Growth Substances Peptides Platelet-Derived Growth Factor
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Childs C B
Proper J A
Tucker R F
Moses H L
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.17.5312
Published
1982-09-00
Pages
5312-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346886
Subset
IM
Grants
NCI NIH HHS · CA 27217 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.17.5312 PMC Full Text View journal details
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