Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 9 ·1980-09-00 ·Pages 5258-62

Todaro GJ, Fryling C, De Larco JE

Abstract

Three different human tumor lines in culture, a rhabdomyosarcoma, a bronchogenic carcinoma and a metastatic melanoma, release proteins (transforming growth factors, TGFs) into the medium that confer the transformed phenotype on untransformed fibroblasts. These proteins are acid and heat-stable; produce profound morphologic changes in rat and human fibroblasts; and enable normal anchorage-dependent cells to grow in agar. Removal of the transforming protein results in a reversion of cell phenotype. The major activity interacts with epidermal growth factor (EGF) cell membrane receptors. The peptides from these tumor cells are similar in their action to the sarcoma growth factor (SGF) released by murine sarcoma virus-transformed rodent cells. The most anchorage-independent tumor cells released the most TGFs. EGF-related TGFs were not detectable in fluids from cultures of cells with high numbers of free EGF membrane receptors (normal human fibroblasts and human carcinomas).

MeSH Terms

Adult Aged Animals Binding, Competitive Carcinoma, Bronchogenic/metabolism Carcinoma, Squamous Cell/metabolism Cell Division Cell Transformation, Neoplastic Cells, Cultured DNA/biosynthesis Epidermal Growth Factor/antagonists & inhibitors,metabolism ErbB Receptors Female Fibroblasts Growth Substances/metabolism Humans Lung Neoplasms/metabolism Male Melanoma/metabolism Peptides/metabolism Rats Receptors, Cell Surface/metabolism Rhabdomyosarcoma/metabolism

Chemicals

Growth Substances Peptides Receptors, Cell Surface Epidermal Growth Factor DNA ErbB Receptors

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Todaro G J

Fryling C

De Larco J E

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20 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.9.5258

Published

1980-09-00

Pages

5258-62

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC350037

Subset

External Links

PubMed source DOI: 10.1073/pnas.77.9.5258 PMC Full Text View journal details

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Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors.

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