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PMID: 3001708 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Type beta transforming growth factor controls the adipogenic differentiation of 3T3 fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 24 ·1985-12-00 ·Pages 8530-4

Ignotz RA, Massagué J

Abstract

Differentiating mouse 3T3-L1 preadipocytes have been used as a model system to study the ability of type beta transforming growth factor (TGF-beta) to modulate cell development. We find that TGF-beta inhibits potently (ID50 approximately equal to 25 pM) the adipogenic conversion of 3T3-L1 cells. Inhibition is observed only when cells are exposed to TGF-beta before they become committed to differentiation. Even a transient (4 hr) exposure to TGF-beta immediately before the commitment point is sufficient to prevent differentiation. This point coincides with the time point immediately preceding the onset of coordinate expression of differentiation-specific proteins in 3T3-L1 cells. TGF-beta interacts with cell surface receptors in 3T3-L1 cells that have structural and binding properties similar to TGF-beta receptors in other cell types in which TGF-beta acts as a growth activator or a growth inhibitor. However, TGF-beta does not markedly alter differentiation-related mitosis in 3T3-L1 cells. The action of TGF-beta on 3T3-L1 cells does not involve changes in cAMP or prostaglandin E levels. These results suggest that TGF-beta is a unique modulator of adipogenic differentiation of fibroblasts.

MeSH Terms
Adipose Tissue/cytology,metabolism Animals Cell Differentiation/drug effects Cell Division/drug effects Cells, Cultured Cyclic AMP/biosynthesis Dose-Response Relationship, Drug ErbB Receptors Glucosephosphate Dehydrogenase/metabolism Growth Substances/pharmacology Mice Multienzyme Complexes/metabolism Oxo-Acid-Lyases/metabolism Peptides/pharmacology Prostaglandins E/biosynthesis Receptors, Cell Surface/metabolism Time Factors Transforming Growth Factors
Chemicals
Growth Substances Multienzyme Complexes Peptides Prostaglandins E Receptors, Cell Surface Transforming Growth Factors Cyclic AMP Glucosephosphate Dehydrogenase ErbB Receptors Oxo-Acid-Lyases citrate (pro-3S)-lyase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ignotz R A
Massagué J
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.24.8530
Published
1985-12-00
Pages
8530-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390950
Subset
IM
Grants
NCI NIH HHS · CA34610 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.24.8530 PMC Full Text View journal details
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