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PMID: 2871553 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Type beta transforming growth factor is the primary differentiation-inducing serum factor for normal human bronchial epithelial cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 8 ·1986-04-00 ·Pages 2438-42

Masui T, Wakefield LM, Lechner JF, LaVeck MA, Sporn MB, Harris CC

Abstract

Type beta transforming growth factor (TGF-beta) was shown to be the serum factor responsible for inducing normal human bronchial epithelial (NHBE) cells to undergo squamous differentiation. NHBE cells were shown to have high-affinity receptors for TGF-beta. TGF-beta induced the following markers of terminal squamous differentiation in NHBE cells: (i) increase in Ca ionophore-induced formation of crosslinked envelopes; (ii) increase in extracellular activity of plasminogen activator; (iii) irreversible inhibition of DNA synthesis; (iv) decrease in clonal growth rate; and (v) increase in cell surface area. The IgG fraction of anti-TGF-beta antiserum prevented both the inhibition of DNA synthesis and the induction of differentiation by either TGF-beta or whole blood-derived serum. Therefore, TGF-beta is the primary differentiation-inducing factor in serum for NHBE cells. In contrast, TGF-beta did not inhibit DNA synthesis of human lung carcinoma cells even though the cells possess comparable numbers of TGF-beta receptors with similar affinities for the factor. Epinephrine antagonized the TGF-beta-induced inhibition of DNA synthesis and squamous differentiation of NHBE cells. Although epinephrine increased the cyclic AMP levels in NHBE cells, TGF-beta did not alter the intracellular level in NHBE cells in either the presence or absence of epinephrine. Therefore, epinephrine and TGF-beta appear to affect different intracellular pathways that control growth and differentiation processes of NHBE cells.

MeSH Terms
Bronchi/cytology,physiology Cell Cycle/drug effects Cell Differentiation Cell Line Cells, Cultured Cyclic AMP/metabolism Epinephrine/pharmacology Epithelial Cells Growth Substances/physiology Humans Lung Neoplasms/pathology Peptides/physiology Receptors, Cell Surface/physiology Receptors, Transforming Growth Factor beta Transforming Growth Factors
Chemicals
Growth Substances Peptides Receptors, Cell Surface Receptors, Transforming Growth Factor beta Transforming Growth Factors Cyclic AMP Epinephrine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Masui T
Wakefield L M
Lechner J F
LaVeck M A
Sporn M B
Harris C C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.8.2438
Published
1986-04-00
Pages
2438-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323313
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.8.2438 PMC Full Text View journal details
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