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PMID: 3470308 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Transforming growth factor-beta increases steady state levels of type I procollagen and fibronectin messenger RNAs posttranscriptionally in cultured human dermal fibroblasts.

The Journal of clinical investigation ·Vol. 79 ·No. 4 ·1987-04-00 ·Pages 1285-8

Raghow R, Postlethwaite AE, Keski-Oja J, Moses HL, Kang AH

Abstract

Transforming growth factor-beta (TGF beta), when injected subcutaneously into newborn mice, induces a rapid fibrotic response, stimulates chemotaxis, and elevates the rates of biosynthesis of collagen and fibronectin by fibroblasts in vitro. We explored the molecular mechanisms of TGF beta-mediated stimulation of collagen and fibronectin synthesis in cultured human foreskin fibroblasts. TGF beta preferentially stimulated the synthesis of fibronectin and type I procollagen chains 3-5-fold as shown by polypeptide analysis. Concomitant elevation in the steady state levels of messenger RNAs (mRNAs) coding for type I procollagen and fibronectin also occurred but without a net increase in the rate of transcription of either of these genes. The preferential stabilization of mRNAs specifying type I procollagen and fibronectin provides a partial explanation for the mechanisms by which TGF beta enhances the synthesis of type I procollagen and fibronectin in mesenchymal cells.

MeSH Terms
Collagen/biosynthesis Electrophoresis, Polyacrylamide Gel Fibroblasts/drug effects,metabolism Fibronectins/genetics Humans Peptides/pharmacology Procollagen/genetics Protein Processing, Post-Translational RNA, Messenger/metabolism Skin/cytology,drug effects Transforming Growth Factors
Chemicals
Fibronectins Peptides Procollagen RNA, Messenger Transforming Growth Factors Collagen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Raghow R
Postlethwaite A E
Keski-Oja J
Moses H L
Kang A H
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30 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI112950
Published
1987-04-00
Pages
1285-8
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC424335
Subset
IM
Grants
NIADDK NIH HHS · AM-16506 · United States
NIADDK NIH HHS · AM-26034 · United States
NCI NIH HHS · CA-42572 · United States
External Links
PubMed source DOI: 10.1172/JCI112950 PMC Full Text View journal details
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