Home LiteratureArticle Details
PMID: 3416833 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A progesterone responsive element maps to the far upstream steroid dependent DNase hypersensitive site of chicken lysozyme chromatin.

The EMBO journal ·Vol. 7 ·No. 7 ·1988-07-00 ·Pages 2063-73

Hecht A, Berkenstam A, Strömstedt PE, Gustafsson JA, Sippel AE

Abstract

We have investigated the influence of the 5'-flanking region of the chicken lysozyme gene on steroid dependent gene expression. By transient transfection of lysozyme-CAT fusion genes into the human breast cancer cell line T-47D, a DNA element was identified which stimulates CAT expression when transfected cells are treated with progesterone. This element is distinct from a second hormone responsive element (HRE) located in the lysozyme promoter region; it activates the lysozyme and the TK promoter, irrespective of orientation and distance, and is therefore referred to as hormone responsive element on its own. The location of this newly discovered HRE between -2250 and -1815 relative to the transcriptional start site, corresponds to the position of a steroid inducible DNase I-hypersensitive site in chromatin of oviduct cells. This observation suggests a physiological role for the upstream element. In vitro DNase I protection experiments revealed six binding sites for both progesterone and glucocorticoid receptors within the sequences of the upstream HRE. The three distal binding sites are not required for hormonal stimulation of the TK promoter, while the three proximal binding sites, which are contiguously arranged, work in a cooperative manner.

MeSH Terms
Animals Base Sequence Cell Line Chickens Chromatin/drug effects Chromosome Deletion Deoxyribonuclease I/pharmacology Enhancer Elements, Genetic/drug effects Genes/drug effects Molecular Sequence Data Muramidase/genetics Mutation Plasmids Progesterone/pharmacology Promoter Regions, Genetic/drug effects Receptors, Glucocorticoid/metabolism Receptors, Progesterone/metabolism Transfection
Chemicals
Chromatin Receptors, Glucocorticoid Receptors, Progesterone Progesterone Deoxyribonuclease I Muramidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hecht A
Zentrum für Molekulare Biologie, Universität Heidelberg (ZMBH), FRG.
Berkenstam A
Strömstedt P E
Gustafsson J A
Sippel A E
References (54)
54 references, click to expand
  1. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  2. Sequences in the promoter region of the chicken lysozyme gene required for steroid regulation and receptor binding.
    Cell. 1984 Jun;37(2):503-10 PMID: 6722881
  3. Hormonal control of egg white protein messenger RNA synthesis in the chicken oviduct.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:617-24 PMID: 277310
  4. Control of cellular content of chicken egg white protein specific RNA during estrogen administration and withdrawal.
    Biochemistry. 1979 Feb 20;18(4):616-24 PMID: 420804
  5. Establishment and characterization of a cell line of human breast carcinoma origin.
    Eur J Cancer. 1979 May;15(5):659-70 PMID: 228940
  6. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  7. Arrangement of coding and intervening sequences of chicken lysozyme gene.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6196-200 PMID: 293713
  8. An improved method for the rapid isolation of brain ribonucleic acid.
    Biochem J. 1979 Oct 1;183(1):181-4 PMID: 534484
  9. Hormone-responsive expression of an endogenous proviral gene of mouse mammary tumor virus after molecular cloning and gene transfer into cultured cells.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2038-42 PMID: 6264458
  10. Multiple mRNAs are generated from the chicken lysozyme gene.
    Cell. 1981 Sep;25(3):743-52 PMID: 7285117
  11. Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary tumour virus chimaeric plasmids.
    Nature. 1981 Nov 19;294(5838):228-32 PMID: 6272123
  12. Transient expression of the chicken lysozyme gene after transfer into human cells.
    EMBO J. 1982;1(10):1207-12 PMID: 6327255
  13. Reversible and persistent changes in chromatin structure accompany activation of a glucocorticoid-dependent enhancer element.
    Cell. 1984 Aug;38(1):29-38 PMID: 6088072
  14. Alternative sets of DNase I-hypersensitive sites characterize the various functional states of the chicken lysozyme gene.
    Nature. 1984 Sep 13-19;311(5982):163-5 PMID: 6236374
  15. Tissue-specific DNaseI hypersensitive sites in the 5'-flanking sequences of the tryptophan oxygenase and the tyrosine aminotransferase genes.
    EMBO J. 1984 Sep;3(9):2015-20 PMID: 6149120
  16. Glucocorticoid and progesterone receptors bind to the same sites in two hormonally regulated promoters.
    Nature. 1985 Feb 21-27;313(6004):706-9 PMID: 2983219
  17. Hormonal response region in the mouse mammary tumor virus long terminal repeat can be dissociated from the proviral promoter and has enhancer properties.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1020-4 PMID: 2983325
  18. Receptors for glucocorticosteroid and progesterone recognize distinct features of a DNA regulatory element.
    Proc Natl Acad Sci U S A. 1986 May;83(9):2817-21 PMID: 3458241
  19. The lysozyme enhancer: cell-specific activation of the chicken lysozyme gene by a far-upstream DNA element.
    EMBO J. 1986 Apr;5(4):719-24 PMID: 3458587
  20. Glucocorticoid responsiveness of the transcriptional enhancer of Moloney murine sarcoma virus.
    Cell. 1986 Jul 18;46(2):283-90 PMID: 3013424
  21. Stoichiometric analysis of the specific interaction of the glucocorticoid receptor with DNA.
    J Biol Chem. 1986 Sep 5;261(25):11770-8 PMID: 3017946
  22. An estrogen-responsive element derived from the 5' flanking region of the Xenopus vitellogenin A2 gene functions in transfected human cells.
    Cell. 1986 Sep 26;46(7):1053-61 PMID: 3463433
  23. Molecular model of the interaction between the glucocorticoid receptor and the regulatory elements of inducible genes.
    DNA. 1986 Oct;5(5):383-91 PMID: 3096677
  24. The hormone regulatory element of mouse mammary tumour virus mediates progesterone induction.
    EMBO J. 1986 Sep;5(9):2237-40 PMID: 3023063
  25. Distinct sequence elements involved in the glucocorticoid regulation of the mouse mammary tumor virus promoter identified by linker scanning mutagenesis.
    J Mol Biol. 1986 Aug 5;190(3):379-89 PMID: 3023641
  26. Identification of estrogen-responsive DNA sequences by transient expression experiments in a human breast cancer cell line.
    Nucleic Acids Res. 1986 Nov 25;14(22):8755-70 PMID: 3466137
  27. In vivo protein-DNA interactions in a glucocorticoid response element require the presence of the hormone.
    Nature. 1986 Dec 18-31;324(6098):686-8 PMID: 2879246
  28. Steroid-dependent interaction of transcription factors with the inducible promoter of mouse mammary tumor virus in vivo.
    Cell. 1987 Jan 30;48(2):261-70 PMID: 3026639
  29. Sequence-specific DNA binding of the progesterone receptor to the uteroglobin gene: effects of hormone, antihormone and receptor phosphorylation.
    EMBO J. 1986 Dec 1;5(12):3235-41 PMID: 3816760
  30. Cooperativity of glucocorticoid response elements located far upstream of the tyrosine aminotransferase gene.
    Cell. 1987 Apr 10;49(1):29-38 PMID: 2881624
  31. Six distinct nuclear factors interact with the 75-base-pair repeat of the Moloney murine leukemia virus enhancer.
    Mol Cell Biol. 1987 Mar;7(3):1101-10 PMID: 3561410
  32. DNase I-hypersensitive sites in the chromatin structure of the lysozyme gene in steroid hormone target and non-target cells.
    Biol Chem Hoppe Seyler. 1987 Feb;368(2):111-9 PMID: 3566913
  33. The hormone response element of the mouse mammary tumour virus DNA mediates the progestin and androgen induction of transcription in the proviral long terminal repeat region.
    EMBO J. 1987 Feb;6(2):363-8 PMID: 3034586
  34. Glucocorticoid induction of the rat tryptophan oxygenase gene is mediated by two widely separated glucocorticoid-responsive elements.
    EMBO J. 1987 Mar;6(3):625-30 PMID: 3582368
  35. Lysozyme gene activity in chicken macrophages is controlled by positive and negative regulatory elements.
    Nucleic Acids Res. 1987 May 26;15(10):4163-78 PMID: 3588288
  36. Interaction of the TGGCA-binding protein with upstream sequences is required for efficient transcription of mouse mammary tumor virus.
    EMBO J. 1987 May;6(5):1355-60 PMID: 3038519
  37. Association of DNA-bound progesterone receptors.
    Nature. 1987 Sep 3-9;329(6134):79-81 PMID: 3041228
  38. Activity of two different silencer elements of the chicken lysozyme gene can be compensated by enhancer elements.
    EMBO J. 1987 Aug;6(8):2297-303 PMID: 3665875
  39. Oestrogen and glucocorticoid responsive elements are closely related but distinct.
    Nature. 1987 Oct 22-28;329(6141):734-6 PMID: 3670376
  40. A DNA sequence of 15 base pairs is sufficient to mediate both glucocorticoid and progesterone induction of gene expression.
    Proc Natl Acad Sci U S A. 1987 Nov;84(22):7871-5 PMID: 2891134
  41. Location and characterization of two widely separated glucocorticoid response elements in the phosphoenolpyruvate carboxykinase gene.
    Mol Cell Biol. 1988 Jan;8(1):96-104 PMID: 3422101
  42. Cooperativity of the glucocorticoid receptor and the CACCC-box binding factor.
    Nature. 1988 Mar 3;332(6159):87-90 PMID: 2831456
  43. Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment whose transcription is regulated by glucocorticoids in vivo.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6628-32 PMID: 6273877
  44. Structure of the lysozyme gene and expression in the oviduct and macrophages.
    Haematol Blood Transfus. 1981;26:175-8 PMID: 7319290
  45. Glucocorticoid regulation of the Ha-MuSV p21 gene conferred by sequences from mouse mammary tumor virus.
    Cell. 1981 Dec;27(2 Pt 1):245-55 PMID: 6277498
  46. Purified glucocorticoid receptor-hormone complex from rat liver cytosol binds specifically to cloned mouse mammary tumor virus long terminal repeats in vitro.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5157-61 PMID: 6291027
  47. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  48. The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6777-81 PMID: 6294651
  49. Expression of a chicken lysozyme recombinant gene is regulated by progesterone and dexamethasone after microinjection into oviduct cells.
    Cell. 1982 Nov;31(1):167-76 PMID: 6297746
  50. Nuclease-hypersensitive sites in the chromatin domain of the chicken lysozyme gene.
    Nucleic Acids Res. 1983 Jun 11;11(11):3467-85 PMID: 6304632
  51. DNA sequences bound specifically by glucocorticoid receptor in vitro render a heterologous promoter hormone responsive in vivo.
    Cell. 1983 Jun;33(2):489-99 PMID: 6190571
  52. The glucocorticoid receptor binds to defined nucleotide sequences near the promoter of mouse mammary tumour virus.
    Nature. 1983 Aug 25-31;304(5928):749-52 PMID: 6310405
  53. Sequence-specific binding of glucocorticoid receptor to MTV DNA at sites within and upstream of the transcribed region.
    Cell. 1983 Dec;35(2 Pt 1):381-92 PMID: 6317184
  54. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-07-00
Pages
2063-73
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454488
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2