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PMID: 3561410 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Six distinct nuclear factors interact with the 75-base-pair repeat of the Moloney murine leukemia virus enhancer.

Molecular and cellular biology ·Vol. 7 ·No. 3 ·1987-03-00 ·Pages 1101-10

Speck NA, Baltimore D

Abstract

Binding sites for six distinct nuclear factors on the 75-base-pair repeat of the Moloney murine leukemia virus enhancer have been identified by an electrophoretic mobility shift assay combined with methylation interference. Three of these factors, found in WEHI 231 nuclear extracts, which we have named LVa, LVb, and LVc (for leukemia virus factors a, b, and c) have not been previously identified. Nuclear factors that bind to the conserved simian virus 40 corelike motif, the NF-1 motif, and the glucocorticoid response element were also detected. Testing of multiple cell lines showed that most factors appeared ubiquitous, except that the NF-1 binding factor was found neither in nuclear extracts from MEL cells nor in the embryonal carcinoma cell lines PCC4 and F9, and core-binding factor was relatively depleted from MEL and F9 nuclear extracts.

MeSH Terms
Animals Base Sequence Binding Sites Cell Line DNA, Viral/genetics DNA-Binding Proteins/metabolism Enhancer Elements, Genetic Genes, Regulator Genes, Viral Mice Moloney murine leukemia virus/genetics,metabolism Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Viral DNA-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Speck N A
Baltimore D
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42 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.3.1101-1110.1987
Published
1987-03-00
Pages
1101-10
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365182
Subset
IM
Grants
NIAID NIH HHS · AI06713 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.3.1101-1110.1987 PMC Full Text View journal details
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